FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3941249
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC833882.1157759887791916No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC670111.700247814842452No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA553401.4041234136691185No Hit
CCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG488351.2390742122611387No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC313180.7946211974934849No Hit
CGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG276840.7024169241780969No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC228220.579055015300987No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT203640.5166889988427527No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT200740.5093309252980464No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA200230.5080369192608739No Hit
GCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGG154780.39271814594814997No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG144170.3657977458414832No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC124380.3155852370657119No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT124120.3149255477134279No Hit
GGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC123000.31208380896512755No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA121480.30822716352100565No Hit
GGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG119930.3042944000746971No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC114500.29051704167891956No Hit
CTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT109240.2771710186288661No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG108780.27600387592867137No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA102000.25880120743449603No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT94370.2394418622116999No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT94340.23936574420951326No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTCAACC94210.23903589953337126No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC87300.22150338636305394No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG84780.21510947417937815No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT84640.2147542568358406No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC73420.18628612401804606No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG70460.17877581446896657No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC66630.1690580828564752No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTCTGAGCAACATGA65840.16705364213222765No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA63840.16197910865311987No Hit
GAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCT63070.16002541326366337No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC62740.1591881152396106No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA62440.15842693521774442No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA62160.15771650053066935No Hit
CTCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG61510.1560672771499593No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA61110.15505237045413775No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA60230.15281957572333033No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA59660.15137333368178463No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG58330.14799876891817798No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA52510.1332318764939744No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTCAACC50590.1283603243540309No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC48220.12234700218128822No Hit
CCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGG48090.12201715750514622No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC46510.11800827605665108No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG45870.1163844253433366No Hit
CGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC45080.11437998461908902No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA42550.10796069976801771No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA41890.10628610371991214No Hit
CCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGG41430.10511896101971735No Hit
CGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG40710.10329212896723855No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGACCTAAACC40510.10278467561932778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC495300.0143.576145
AGTTGTC214550.0142.29256145
CGTGCTA77800.0141.92072145
CGTGCCC167650.0140.97415145
CGAACGA208400.0140.826631
GAACGAT412300.0138.794022
GGAACGA46500.0137.829221
CGTACTG479250.0137.26572145
AACGATT395550.0136.643263
AGTGCTC395350.0136.52382145
TGAACGA182000.0136.357031
ACCGATT18000.0134.529023
CGTTGTC127400.0133.84229145
GGTCACA47350.0133.81874145
GGTGCTC146150.0133.2394145
CCGGTTC27750.0132.98321
ACGATTC427700.0132.576034
GGTGCCC48800.0132.51666145
GGTCACC490950.0132.08957145
CGTGCGC45300.0131.5525145
Produced by FastQC (version 0.11.5)