FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849078
Sequences flagged as poor quality0
Sequence length114-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT2322423.3908505641197255No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1915262.7963763881795476No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1742712.5444446683188597No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1489782.1751540864332397No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC1177451.7191365027526333No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1145131.6719476694527349No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG974241.422439633480594No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG922411.3467652142375952No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG912681.3325589225294265No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT868661.2682874979668797No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC833251.2165871085129998No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC825201.2048337017040833No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT731801.068464981709947No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT709961.036577478019669No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG705901.0306496728464767No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT595840.8699565109347565No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC580410.8474279311755539No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC447030.6526863907813577No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT432490.6314572559985446No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC424460.6197330502003335No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT417670.6098193070658562No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA403680.5893932000774411No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT387580.5658863864596081No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG361710.5281148791121958No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG350790.5121711272670569No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG326560.4767941027974861No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT304290.4442787773770425No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA299870.4378253540111531No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG292250.4266997689324023No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT291460.42554632900953965No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC283430.4138221232113286No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC280650.4097631827232804No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG274230.4003896582868526No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG273070.39869599966594044No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT268800.39246158388034125No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG232920.340074970674885No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT227130.33162127807567676No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG219720.32080230360933254No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT219000.31975106722393876No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT213250.3113557766461413No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC205070.2994125632676398No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC192040.2803881047930831No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC190570.27824183050623746No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA189840.277175993615491No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC179220.26167025693093293No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA179180.2616118549095221No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT178120.2600642013421368No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG174690.2550562280061638No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT172000.2511286920662898No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT171620.2505738728628875No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG171150.24988764911131103No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC170710.24924522687579262No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT170400.24879261120985918No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT168760.24639812833201782No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC163820.23918547868778833No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG161050.23514113870509284No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT160710.23464472152310137No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG159750.233243073009243No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG159190.23242544470949228No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC158930.23204583157032233No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC158100.23083398962604895No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC156480.22846870775891295No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT156460.22843950674820757No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT153410.22398635261563676No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT150870.22027782425605313No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT145980.21313817713858713No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG144120.21042248314298656No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG142200.20761918611526983No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT140300.2048450900982585No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC138030.20153077538319755No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA135650.19805585510925702No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC133440.19482914342631227No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT132800.19389471108374004No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC132540.19351509794457006No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC132300.19316468581610546No Hit
GCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC131710.19230325600029669No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA130490.19052199434726835No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT130210.19011318019739298No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC127630.18634624981639863No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC125740.18358675430474No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT124890.18234571134976124No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT123600.18046224615926407No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC123180.17984902493445104No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA120230.1755418758554071No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC118130.17247576973134193No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC118090.17241736770993119No Hit
GGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC117800.1719939530547031No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC117700.1718479480011762No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG117340.17132232980847933No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG115900.16921985703769177No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT115660.1688694449092272No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC115600.16878184187711104No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG113070.16508791402288014No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG112460.16419728319636598No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC111330.16254742609151188No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC110750.1617005967810558No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT107220.15654661839155576No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC104380.15240007487139143No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT104240.15219566779645377No Hit
GGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGGTACCAGTC103740.15146564252881922No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA103520.15114443141106No Hit
GAGAGAAGGAACTGTGTCACCACAGAAACTTCTGCCTTCACCCATCCCTT103400.1509692253468277No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC103080.1505020091755416No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG101870.14873534802786595No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG98990.14453040248629084No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT97920.14296814841355288No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG97230.1419607135442172No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC97110.1417855074799849No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG96250.14052986401965345No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC95120.13888000691479932No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT94800.13841279074351323No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT89250.13030951027276957No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG89060.1300321006710684No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG88450.12914146984455427No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT88230.12882025872679503No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC85580.12495112480833187No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC84100.12279025001613357No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC83150.12140320200762789No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT83030.1212279959433956No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG82910.1210527898791633No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC82220.12004535500982759No Hit
GCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGC82170.11997235248306413No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC81830.11947593530107264No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA81630.11918392519401882No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA81560.11908172165654997No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC79580.11619082159671712No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC79160.11557760037190408No Hit
ACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGG78310.1143365574169253No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT77930.11378173821352305No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG77770.11354813012788No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA76050.11103684320721709No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG75480.11020461440211368No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT74330.10852555628655419No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTG71790.1048170279269706No Hit
GCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTG70980.10363438699340262No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC70910.10353218345593378No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT70120.10237874353307118No Hit
GCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCC69720.10179472331896351No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT69560.10156111523332044No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC69490.10145891169585163No Hit
GAGAGACTCCAGGATCATCCACTGAGCACTGGACATAAGGAAGGCTGCAT69190.10102089653527088No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG68860.10053907985863207No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT68680.10027627076228364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG31900.0111.3431851
GCTTCAA80850.0109.791891
GAGTGAT82400.0109.728181
GGATCTG124450.0108.955071
GACTACG19450.0108.1149141
GCTATTT16350.0108.07881
GAGAACT31900.0107.465561
GAGAGAC24650.0107.052821
GAGAGCT70000.0106.866781
GAGTTAA9100.0106.81485118
GCTTTTC108150.0105.769321
GTCTTTC15350.0105.1427151
GACTGTA3650.0103.2818761
GCACTAC6800.0103.080151
GACTGCA9150.0102.999691
GGTTACC54750.0101.94061118
GGCTGTT44750.0101.2209241
GCCCGAC4950.0101.146481
GATCCCT10100.0100.309971
AGTGGTA156550.0100.271612
Produced by FastQC (version 0.11.5)