FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5025214
Sequences flagged as poor quality0
Sequence length113-123
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT1655413.2942079680586738No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1143062.2746493980156863No Hit
CAAGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACAGGACACATG516381.0275781290110233No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT367590.7314912359951238No Hit
CAGACTGAGAACCACCGCTATATGTACTGGTATCGACAAGACCCGGGGCA319870.6365301059815561No Hit
GGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTG317590.6319929857713522No Hit
CATGGACTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCTGGTAGCAA317510.6318337885709943No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC313330.623515734852287No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT289100.5752988827938472No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC256740.5109036152490223No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC255620.5086748544440097No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA211030.4199423148944502No Hit
CAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAACAGTGAC200710.3994058760482638No Hit
AAGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACAGGACACATGG184170.3664918548742402No Hit
CCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTATATGTACTG180110.3584125969560699No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG172710.34368685592295173No Hit
GGACACATGGATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGA168720.33574689555509474No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT168610.3355279994046025No Hit
TCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG165080.3285034229388042No Hit
TGGACTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCTGGTAGCAAAG160150.31869289546674034No Hit
CATGGGAATGACAAATAAGAAGTCTTTGAAATGTGAACAACATATGGGGC156360.3111509280997784No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG152580.3036288603828613No Hit
CCTTTGTCTCCTGTGGACAGGACACATGGATGCTGGAATCACCCAGAGCC148170.2948531147131247No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA143850.2862564658937908No Hit
TCCAATTTCAGGTCATACTGCCCTTTACTGGTACCGACAAAGCCTGGGGC132830.2643270515444715No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC132680.2640285567938002No Hit
CAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAAGTCTTTGAA132460.26359076449281565No Hit
TGGATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGA131910.2624962837403541No Hit
AGATCACGCAGATACTGGAGTCTCCCAGAACCCCAGACACAAGATCACAA130980.260645616286192No Hit
GATGTCACCAGACTGAGAACCACCGCTATATGTACTGGTATCGACAAGAC128030.2547752195229895No Hit
AGATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGAC124460.24767104445701216No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG124040.2468352591551325No Hit
GAGTCTCCCAGTCTCCCAGGTACAAAGTCACAAAGAGGGGACAGGATGTA121630.24203944349434672No Hit
GGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT121590.2419598448941677No Hit
CAGGACACATGGATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACA119060.23692523343284486No Hit
CACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCCTGGGGGCAGATCACA117680.23417908172666876No Hit
GAGACAGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCG117320.2334626943250576No Hit
TAGCAAAGCACACAGATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTG116550.2319304212716115No Hit
CCGCTATATGTACTGGTATCGACAAGACCCGGGGCATGGGCTGAGGCTGA111930.22273678295093502No Hit
CACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCCTGGGGGCAGATCACG110180.21925434419310302No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT108020.2149560197834361No Hit
CAGATCACGCAGATACTGGAGTCTCCCAGAACCCCAGACACAAGATCACA105870.2106775950238139No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG105500.20994130797215801No Hit
AAAGCACACAGATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTGACAG102320.2036132192579261No Hit
CATGGACTCCTGGACCTTCTGCTGTGTGTCCCTTTGCATCCTGGTAGCGA100470.19993178399964656No Hit
AGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCCTCTGCT98210.1954344630895321No Hit
CTGAGATGTAAACCAATTTCAGGACACGACTACCTTTTCTGGTACAGACA98080.19517576763895028No Hit
GAAGTTACCCAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAA97880.1947777746380552No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC97610.19424048408684683No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC97490.1940016882863098No Hit
CTCTGCCATGGACTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCTGG97280.19358379563536995No Hit
GGAGTCTCCCAGTCTCCCAGGTACAAAGTCACAAAGAGGGGACAGGATGT96100.19123563693008894No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA94600.1882506894233758No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGGGCACCAGTC91310.18170370455865165No Hit
CAGGGATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCG89610.17832076405104338No Hit
GGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCATGTATCCCTTTATT88220.17555471269482253No Hit
GGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCC88160.17543531479455404No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC87300.17372394489070514No Hit
AGACTGAGAACCACCGCTATATGTACTGGTATCGACAAGACCCGGGGCAT86890.17290805923887023No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG83860.16687846527530967No Hit
AGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACAGGACACATGGA82150.16347562511765668No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA80370.15993348740969043No Hit
AAGCACACAGATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTGACAGA78980.15716743605346956No Hit
CTCCAGGCTGCTCTGTTGGGTGCTGCTTTGTCTCCTGGGAGCAGGCCCAG78680.15657044655212693No Hit
AGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGACAAGAAGTGA77350.1539237930961746No Hit
TCCAATTTCTGAACACAACCGCCTTTATTGGTACCGACAGACCCTGGGGC77020.1532671046446977No Hit
TGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACAC76820.15286911164380262No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC74310.1478742994825693No Hit
GGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGT74290.1478345001824798No Hit
TGCTGCTTTGTCTCCTGGGAGCAGGCCCAGTAAAGGCTGGAGTCACTCAA73400.14606343132849664No Hit
CAGCCTCCTCTGCTGGATGGCCCTGTGTCTCCTGGGGGCAGATCACGCAG73310.14588433447809387No Hit
GATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCATGTATCCCTTTATTG72500.14427246282446876No Hit
AGCAGTTCCCATAGACACTGAAGTTACCCAGACACCAAAACACCTGGTCA71950.14317798207200727No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC71920.14311828312187302No Hit
CAATTTTTAGTCTCTTGAAAGCAGGACTCACAGAACCTGAAGTCACCCAG71740.14276008942106744No Hit
GAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGG70760.14080992371668152No Hit
CACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCTAGGGACAGATCACA70610.14051142896601018No Hit
CAACATATGGGGCACAGGGCTATGTATTGGTACAAGCAGAAAGCTAAGAA69830.13895925626251937No Hit
ACAGAATGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGT69360.13802397271041592No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC68990.13728768565876No Hit
GATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTGACAGAGATGGGACA68530.13637230175670131No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC67000.13332765529985388No Hit
AGTCACTCAAACTCCAAGATATCTGATCAAAACGAGAGGACAGCAAGTGA66350.13203417804694487No Hit
GATCACGCAGATACTGGAGTCTCCCAGAACCCCAGACACAAGATCACAAA65880.1310988944948414No Hit
GAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTCTGTGTTCAGTGCTGTCA65830.13099939624461762No Hit
AGTGACTTGTTCTCAGAATATGAACCATGAGTATATGTCCTGGTATCGAC65810.13095959694452813No Hit
GCAGTTCCCATAGACACTGAAGTTACCCAGACACCAAAACACCTGGTCAT65340.13002431339242468No Hit
ACAGAATGTAACTTTCAGGTGTGATCCAATTTCTGAACACAACCGCCTTT64800.12894973229000795No Hit
CATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCCTGGGGGCAG63980.12731796098633807No Hit
AAGTCACAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCG63870.1270990648358458No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG63660.12668117218490596No Hit
ACAGGACACATGGATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCAC63480.12632297848410037No Hit
CAAGTGACACTGAGCTGCTCCCCTATCTCTGGGCATAGGAGTGTATCCTG63470.12630307883405564No Hit
AGGTCACAGAGAAGGGAAAATATGTAGAGCTCAGGTGTGATCCAATTTCA63040.1254473938821312No Hit
ACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTACCTTT62770.12491010333092283No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT62420.12421361557935642No Hit
CCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCCTGGGGGCAGATCACGCA62170.12371612432823756No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC61140.12166646037362787No Hit
AGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCGAGTGA60570.12053218032107688No Hit
GGAGTCTCCCAGACCCCCAGTAACAAGGTCACAGAGAAGGGAAAATATGT60410.1202137859203608No Hit
CTGAGATGTCACCAGACTGAGAACCACCGCTATATGTACTGGTATCGACA60310.12001478941991327No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG59810.11901980691767554No Hit
AGCAGGCCCAGTAAAGGCTGGAGTCACTCAAACTCCAAGATATCTGATCA59440.11828351986601963No Hit
CATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCCTGGGAGCAG59040.11748753386422947No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC58380.11617415696127567No Hit
CACAGAGACAGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACC57200.11382599825599468No Hit
CATCTTGATTTAAAAAAGGAAAATTATAATTAGAAAAAGTCAATTTAGTT57130.11368670070568139No Hit
GAGAAGGGAAAATATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCATAC57100.11362700175554712No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC56290.11201513010192202No Hit
AGATCACACAGGTGCTGGAGTCTCCCAGACCCCCAGTAACAAGGTCACAG55930.11129874270031088No Hit
ATGAAAATATGTTCTGGTATCGACAAGACCCAGGTCTGGGGCTACGGCTG55320.11008486404758086No Hit
CTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAACACCA54920.10928887804579068No Hit
AGATGCTTAGTCCTGACCTGCCTGACTCTGCCTGGAACACCAGGCTCCTC54450.10835359449368723No Hit
GAAGTAGGTAGGCAAGTTTTTATTGATAGAGAGGAAATCAAATAATGGCA54250.10795560149279215No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG54030.10751780919180756No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC53760.10698051864059918No Hit
ATGACAAAATGTACTGGTATCAACAAGATCCAGGAATGGAACTACACCTC52700.10487115573585522No Hit
GCTGGTGTCACTCAGACCCCAAAATTCCAGGTCCTGAAGACAGGACAGAG52690.10485125608581047No Hit
GGTGCCATGGGCTCCAGGCTGCTCTGTTGGGTGCTGCTTTGTCTCCTGGG52640.10475175783558671No Hit
GATCACACAGGTGCTGGAGTCTCCCAGTCTCCCAGGTACAAAGTCACAAA51740.10296078933155883No Hit
ATAGACACTGAAGTTACCCAGACACCAAAACACCTGGTCATGGGAATGAC51020.10152801452833651No Hit
CATGGGCTCCAGGCTGCTCTGTTGGGTGCTGCTTTGTCTCCTGGGAGCAG50810.10111012187739665No Hit
CATGGGCACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCTAGGGACAG50740.10097082432708337No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA50510.10051313237605403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)