FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117467
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA2202074.30304680030179No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG897351.7535042238670029No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG890301.7397278770923192No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA534651.044755149373704No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG519091.0143494818823453No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA450520.8803574111958123No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT422430.8254669741885976No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG409860.8009040410030979No Hit
CGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA360900.7052317093593373No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT307150.6001992782757564No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC294200.5748937902286424No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC286220.5593001381347452No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT259690.5074580842436307No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG250270.48905054004256404No Hit
TGGTTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGG233420.45612409420519956No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA228550.4466076674260918No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA211140.412586930213717No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT208660.40774078269581415No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG203740.39812665132965197No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG196710.38438938638979014No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG195580.38218126272235853No Hit
CGAGGATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTG190480.3722153948428002No Hit
TAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG189920.3711211034677898No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG180090.3519123816528763No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT177350.34655817028229No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC162410.31736403967040727No Hit
GAATGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC162090.3167387303132585No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG154180.3012818646412375No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC149550.2922344198799914No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACAGAGC148770.2907102283219413No Hit
TTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGT144570.28250304300936385No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC141580.2766603087035051No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC137000.2677105685293134No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA130250.2545204492769568No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT123310.240959052593793No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA117230.2290781748079665No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA116020.22671372380124777No Hit
AGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA115230.22516999132578674No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA114600.22393891352890014No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC114280.22331360417175136No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC113320.2214376761003051No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC111010.21692372417838746No Hit
GGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA107430.2099280757452857No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA103780.20279564089030763No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA103090.20144731758895562No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC99660.19474478291701736No Hit
CAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA98910.19327921411119992No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT92420.18059715871152662No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG91680.17915113082312012No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT85830.16771969413774432No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG83110.16240456460197983No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA82330.16088037304392974No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA82050.16033322735642458No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT81740.15972745891668672No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAA78810.15400197011529335No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG76040.14858913599247442No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG75450.1474362218652314No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT73840.14429013416207667No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA73300.14323492462188814No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGCTTAATCCCATG73030.14270731985179386No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG72600.14186706040312522No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC71810.14032332792766422No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG69090.13500819839189973No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG68360.13358171142090414No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA68020.13291732022893357No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT66110.1291850050034519No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC65920.12881372757264484No Hit
CAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAGGCC65330.1276608134454018No Hit
GGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTGCTG64690.12641019473110426No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG62800.1227169613404444No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT61500.12017664207702755No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT59800.11685468611717477No Hit
CCCTAACTCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG58790.11488105345867398No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC58320.11396263034036175No Hit
CTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG58240.11380630300107455No Hit
CTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT58000.11333732098321297No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG57540.11243843878231165No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA57310.11198899768186098No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG56460.11032801970193457No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT56290.1099958241059493No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT56140.1097027103447858No Hit
CAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG56060.10954638300549863No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC55680.10880382814388447No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG55170.10780724135592862No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG55080.10763137309923053No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA54990.10745550484253245No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC54490.10647845897198752No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC54030.10557957677108616No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT54000.10552095401885346No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC53480.10450482631348672No Hit
TGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG52550.10268752099427314No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA51900.10141736136256473No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC51670.10096792026211404No Hit
GGGTTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGG51310.10026444723532169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)