FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552653
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA1435243.1525354557002254No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA1235052.7128138252574927No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC1115992.4512959806073513No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC1109692.4374578954293242No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC676451.4858369394724351No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT503871.1067612664527695No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA489981.0762515834174051No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT461771.01428771312024No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT432650.9503250082973598No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC392430.8619809153036703No Hit
GAGTGGATTTGTCATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACAT317220.6967805365355102No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC316320.6948036672243635No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT311190.6835355121508273No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC300060.6590882283363129No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG299280.6573749415999858No Hit
CAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGCCAGAAGACTCAG279090.6130271733865946No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA236160.5187305072448966No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA221080.485606963675905No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG219960.4831468596442558No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA213930.4699018352595728No Hit
CAATGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGA212410.46656312264519173No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT211000.46346602739106185No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC200020.439348221795072No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT199580.438381752354067No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC192390.4225887630794616No Hit
CGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC189820.4169437029354093No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC187860.4126385208800231No Hit
CACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG187040.4108373732854228No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG177110.3890259152191041No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG173560.38122826404735877No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG169550.3724202130054718No Hit
CGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAGAGC142810.3136852292498462No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC130610.2868876674765241No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA128030.28122064211790354No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC125090.27476286903482433No Hit
GAATGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC119180.2617814272249609No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG113390.2490635679899171No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG104850.23030527474859164No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG102880.2259781274786372No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG100640.22105791941533873No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC99430.2184001284525748No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA97030.2131284769561836No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC92640.20348574776070127No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT90910.19968576564038595No Hit
CTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGCTGCTC85000.18670432383052254No Hit
TCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA84780.18622108911002003No Hit
CGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA83960.18441994151541968No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG81220.17840147272370638No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA80620.17708355984960858No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG80460.17673211641651582No Hit
TAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGGACT79970.17565582090266926No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC77420.1700546911877536No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC75430.16568361348866253No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG75130.16502465705161365No Hit
CAGTGGATTTGTCATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACAT75110.16498072662247704No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG74990.1647171440476575No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG74780.16425587454172327No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA74680.16403622239604027No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA72410.1590501186890369No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT70580.1550304844230386No Hit
GGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC70470.15478886706278733No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA70170.15412991062573844No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA70090.15395418890919207No Hit
TTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCAC69760.15322933682843826No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG68240.14989062421405716No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA67890.14912184170416679No Hit
GGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA65880.14470683357593914No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG62700.13772189534322074No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA58370.12821095743514827No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC55850.12267572336393748No Hit
AGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC55310.12148960177724945No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA54340.11935897596412465No Hit
CGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC53570.11766765444236581No Hit
GGTCTCTAGAGCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA52490.11529541126898975No Hit
GGTCTCTAGATCAAAGACAGAGGAGTTCCTCCTCACTCTGGAGTCCGCTA52050.1143289418279847No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG51850.11388963753661875No Hit
TAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG51380.11285727245190881No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT47160.10358795190408758No Hit
CCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA46180.10143536087639449No Hit
GCCCCAGAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGA45590.10013941321686498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA643600.0144.35054145
GGTCACA557650.0144.21976145
AGTTGTA479950.0143.67691145
CGTACTG156850.0141.82423145
ATTTGTC92200.0141.806927
TTGTCAT96600.0141.418219
AAGGGTA49650.0141.137633
CGTTGTC73500.0140.54295145
CATCCTA223100.0140.30028145
GGTGCTC695700.0140.15804145
CGTGCTC135600.0140.02676145
GATTTGT91350.0139.71716
CGTTGTA191850.0139.26906145
CCCTGGT82200.0138.877621
CCGATTC47300.0138.809311
AGTGGAT89650.0138.402042
AGAGCGA83350.0138.27648
GCTATAG47900.0137.987936
TGTTGTA64500.0137.93185145
CTGGTCG84350.0137.573623
Produced by FastQC (version 0.11.5)