FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6406473
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1924513.004008601924959No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG1307942.04159137172669No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA1262611.9708348103550895No Hit
CGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA706591.1029313633258113No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG519380.8107112915327982No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC412680.644160991547143No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA379810.5928535092554046No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC369800.5772286872979876No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA333160.5200365318015077No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG322140.5028351793568786No Hit
TGGTTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGG315320.49218969626501197No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG310880.4852592058063774No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT305880.47745459943404117No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA301250.47022753393325784No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC296210.46236049070994295No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT291070.4543373553591813No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT285700.44595520811529216No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT273310.42661539352464295No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG260910.4072599697212491No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT255220.3983783276695305No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA227420.354984716239341No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC222950.34800739814247245No Hit
TAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG220300.34387095676513424No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC214600.33497370550067096No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG204080.31855281369327554No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG202380.31589924752668125No Hit
AGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA193230.3016168178653059No Hit
TTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGT193100.30141389809962515No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG191160.29838571082715876No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG189870.29637212238309596No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC185950.2902533109871844No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC182310.2845715575481236No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA170830.2666521813172396No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG168820.26351472955556043No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA161820.25258828063428973No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC155220.2422862002228059No Hit
CGAGGATTTCCTCCTCACTCTGGAGTCCGCTACCAGCTCCCAGACATCTG147610.23040758932411018No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA147580.23036076168587616No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA135170.21098972866973761No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC134510.20995952062858925No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG133260.20800836903550518No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC131420.20513627389048544No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT131350.20502700940127275No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC127940.19970426785533946No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT125430.19578635545642667No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG122200.19074457973989745No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA121470.18960510720953636No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA116250.18145709815681732No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT112210.17515097620796966No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT110210.17202913365903516No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG108650.16959409647086626No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG106210.16578544856116617No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC106020.16548887351901742No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA105710.16500498792393256No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACAGAGC101990.1591983607829144No Hit
GGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA99940.15599847217025656No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC98610.1539224468752151No Hit
GAATGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC97480.15215860583506713No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA97260.15181520315468433No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA96230.1502074542419831No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG94220.1470700024803039No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA92140.143823286229412No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT91240.1424184570823915No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA91160.14229358338043413No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT90000.14048291470205213No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC89490.13968684485207383No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA89350.1394683158736484No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC89320.1394214882354144No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAA86600.1351757823688635No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG86120.13442654015711922No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA85880.13405191905124708No Hit
CCCTAACTCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG84770.13231929643658843No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT84180.13139835288465276No Hit
GGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTGCTG82490.1287603959308031No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG80370.12545124282893255No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA80280.12531075991423052No Hit
CTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT77070.12030020262319063No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC76140.1188485458379361No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG75250.11745932590366025No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC74380.11610132439487375No Hit
TGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGCTG73000.11394725303610896No Hit
CAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG72900.11379116090866222No Hit
GGGTTATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGG71210.11115320395481258No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCACTTCCCTGG71160.11107515789108922No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC70970.11077858284894045No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT69090.10784405085294202No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT68680.10720407313041046No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT68570.10703237179021906No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA68150.1063767848549428No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA66810.1042851503471567No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA65660.10249009088151936No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC64800.10114769858547754No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA64520.1007106406286267Illumina RNA PCR Primer (96% over 28bp)
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG64150.10013309975707382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA1284250.0144.25601145
GGTCACA763750.0142.4898145
CCCTAAA212350.0141.143651
CGTACTG409000.0141.12083145
CGTGCTC273300.0139.92842145
CATCCTA267600.0139.25642145
GGTGCTC374050.0138.76376145
AGTGCTC974250.0138.55296145
CGTTGTA221850.0138.14526145
AGTTGTC116550.0137.5969145
AGAGCGA306450.0137.523968
GAGCGAT302900.0137.444059
AGTTGTA352350.0135.10414145
GGGTTGC104100.0134.929959
GAGAGCG310200.0134.880117
CGTGCTG101800.0132.71225145
TGTTGTA72450.0130.61238145
GCTGGGA296650.0130.172292
CCGATTC51850.0130.13091
TGGGAGA298950.0129.993134
Produced by FastQC (version 0.11.5)