FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032162
Sequences flagged as poor quality0
Sequence length112-123
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1995892.838230973632291No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC1430412.0340970529404756No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1332531.8949079955780312No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1310111.8630259086750276No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1271331.8078792837821427No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1178751.6762270266242445No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC1174831.6706526385484293No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1174491.6701691457051189No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG860941.2242892015286337No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC852741.2126284917782042No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT742051.05522313052515No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC676640.9622076396988579No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG666050.9471482596675105No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG611150.8690783858506104No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG545420.7756078429364965No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT512170.7283250869362793No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT484310.6887071145403079No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT479440.6817817905787722No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT463320.6588585416547571No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT400050.5688862116657722No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA385100.547626746937855No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC370390.526708571275804No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC361660.5142941815049198No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC345650.4915273567360934No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG342010.48635113923712225No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG324200.4610246464742991No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT307030.43660825788711916No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG301910.4293274244819729No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG279080.39686230209144785No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC272950.38814521053411455No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC268410.381689159038145No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT267890.3809496993954348No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC265330.37730928269286174No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC261710.3721615059493794No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT254560.36199393586211465No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA246000.3498212925128858No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC242210.3444317693477483No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT241140.34291018892909464No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG234700.33375226566168414No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG233760.33241555015370805No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG228810.32537646317021707No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC227440.32342827141923064No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC220790.3139717202191872No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC217070.3086817396982606No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG213850.3041027780645554No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG209420.29780315072377456No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG208390.2963384518160987No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC205510.2922429830257039No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG205150.2917310494269046No Hit
GAGAGAAGGAACTGTGTCACCACAGAAACTTCTGCCTTCACCCATCCCTT204200.2903801135411841No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT199520.2837249767567926No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA188860.2685660540812342No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC180550.25674892017561596No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC177070.25180022872055563No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT173800.24715016519812824No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG172870.24582767006789663No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG171310.24360929113976612No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA168180.2391583129057607No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT163920.2331004319866351No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT159790.22722741597818708No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC159440.22672970275713217No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT159190.22637419331352152No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT158240.22502325742780102No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC153510.2182970187546874No Hit
GGAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG153130.2177566444003992No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT147290.20945194379765425No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT138060.19632653513954884No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT136430.19400861356720736No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC136350.19389485054525196No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC133570.18994158553230145No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA133010.18914524437861358No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG128970.18340021176986537No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG128780.18313002459272126No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC124740.17738499198397306No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT124400.17690149914066258No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT119820.17038856613371534No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC118700.1687958838263396No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC118650.16872478193761747No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC117460.16703255698603076No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC116810.16610823243264305No Hit
GAGAGACTCCAGGATCATCCACTGAGCACTGGACATAAGGAAGGCTGCAT115040.1635912255718796No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC114980.16350590330541306No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT112700.16026365717968385No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC111510.15857143222809714No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC111020.15787463371862026No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC110590.15726315747560993No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA110540.15719205558688779No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC109020.155030558169735No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT108320.15403513172762517No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT106660.1516745490220504No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC106470.1514043618449063No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG106270.15111995429001776No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG105940.1506506818244517No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG105590.15015296860339677No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG105230.14964103500459744No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC104450.1485318455405322No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC101630.144521699016604No Hit
GACCCATCCCTTCAGCTCTGCAGGACAGGTAGAGACTCCAGGATCATCCA100040.14226065895524023No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA99920.14209001442230712No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC99130.14096660458049742No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC98970.14073907853658663No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT98260.13972943171673236No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT98100.13950190567282153No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG94070.13377109344181776No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC94040.13372843230858447No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC93310.13269034473324134No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG92710.13183712206857578No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT92140.13102656053714348No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT92090.13095545864842134No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT90880.12923479294134577No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA89480.1272439400571261No Hit
GGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT89440.1271870585461484No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT86650.12321957315545348No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC85380.12141358518191134No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC85120.12104385536055626No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG84280.11984934363002446No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG84130.11963603796385806No Hit
GAGGACAGGTAGAGACTCCAGGATCATCCACTGAGCACTGGACATAAGGA83060.11811445754520444No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG82840.11780160923482708No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT82310.11704792921437249No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA80910.1150570763301528No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA80180.11401898875480969No Hit
GTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTC79790.11346439402277707No Hit
GCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC79450.11298090117946658No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT78850.112127678514801No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG78540.11168684680472377No Hit
GGAGAGTACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCC78460.11157308378276837No Hit
GGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGG77800.11063453885163624No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA77120.10966755316501525No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG76100.10821707463508379No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT76000.10807487085763952No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC73790.10493216737612131No Hit
GAGCTCTGCAGGACAGGTAGAGACTCCAGGATCATCCACTGAGCACTGGA72530.1031403997803236No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG72120.10255736429280213No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT70820.10070871518602673No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG70710.10055229103083803No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC70470.10021100196497179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG10700.0103.7020651
GGATCTG103400.0103.6978451
GACTGTT8450.0102.978641
GAGAGCT138500.0102.633321
GCCCGAC6050.0102.3217851
GACTGCA7900.0102.0163041
GCTTCAA48800.0101.004381
GCTTTTC185350.099.849811
GAGAACT33550.098.34981
GCTATTT12100.097.0126341
GAGAGAC31900.095.9308241
GTCTTTC18550.095.0781251
GGTTCAC16200.094.810911
GACTGTA4200.094.553411
GACTACT14550.094.324051
GATCACT38000.093.59471
GTCCGCA251.893807E-593.4410251
GCGTGCA251.893807E-593.4410251
GGAGAGT43650.092.584741
GACAGAC221550.091.996491
Produced by FastQC (version 0.11.5)