FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5325921
Sequences flagged as poor quality0
Sequence length114-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT2002343.759612656665392No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1803863.386944718106033No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG890061.671185133989032No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT806951.5151370063506386No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT739551.3885861243529523No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT646681.2142125277487217No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG631841.1863488023949285No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT601951.1302270536870525No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG488580.9173624618164632No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT443210.8321753176586735No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC442940.8316683630868728No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT440250.8266175934641163No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA422250.7928206220107283No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC364750.6848580743124053No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG345130.6480193754282123No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG341420.6410534440897641No Hit
GGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAG337320.6333552450364922No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC336900.6325666490359132No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG305490.573590933849751No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC294510.5529747812631843No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA288420.541540139254788No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT284020.5332786573439598No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG275070.5164740520935253No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT267920.5030491439884295No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG248140.4659100275802063No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC245550.46104701890996885No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG245230.46044618386190855No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT239590.449856466139847No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA238110.44707760404256836No Hit
GACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAG229830.4315309971740099No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC228440.4289211199339983No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC220260.41356227401795853No Hit
GAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTC214630.4029913323911488No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT211220.39658868391025703No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC210120.39452331343255No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC198680.3730434604643967No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC190920.35847321054893605No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC182190.3420816793940428No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC180980.33980977186856504No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT178940.3359794484371811No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT175450.3294265911942742No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGC175440.32940781509902234No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG175200.32895718881297714No Hit
GAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCT174810.32822492109815377No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT172080.3230990470943899No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC170920.32092102004517153No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG166890.313354253658663No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA162380.3048862347000641No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT162330.3047923542238047No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC159850.3001358826013379No Hit
GAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG153530.2882693904021483No Hit
GGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC151130.2837631275416965No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC148750.27929441687174855No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT140920.26459273428952473No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG138820.2606497542866295No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC138670.2603681128578512No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC137090.2574014898080539No Hit
GACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAGCCTCCT136650.25657534161697104No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG136310.255936954378407No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG136090.25552388028286566No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG135960.25527979104459114No Hit
GCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTG135350.25413444923422635No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT134300.2521629592327787No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT132300.24840774018240228No Hit
GACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCT132160.2481448748488759No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACC129680.2434884032264091No Hit
GGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCCCTCTGTCTCC128140.24059688455761924No Hit
GGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATC124510.23378116198118598No Hit
GAGACCCCAAAATTCCGCATCCTGAAGATAGGACAGAGCATGACACTGCA124360.23349952055240772No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG117720.22103219330515791No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATG117480.22058156701911277No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA117360.22035625387609017No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT114830.21560590177736394No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT113650.21339032253764184No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGGCCTGACCCTGCCAT113610.21331521815663432No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC112040.2103673712020888No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC111470.20929713377273151No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC109340.2052978254840806No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC109120.2048847513885392No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC106270.19953356424175275No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC104820.19681103043022982No Hit
GGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTG104100.1954591515720943No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG104010.19529016671482735No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT101470.19052103852084926No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT93490.1755377145098472No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG92370.1734347918416364No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC91370.17155718231644818No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC90730.17035551222032772No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC89560.1681587090758575No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC89500.1680460525043462No Hit
GACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTG87000.16335202869137563No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG80570.15127899944441534No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGGGCACCAGTC80010.15022753811030992No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG79710.14966425525275345No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG78610.1475988847750464No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG78430.14726091506051253No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT77610.1457212752498582No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG76920.1444257246774783No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC75810.1423415781045194No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC75400.14157175819919224No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT72920.13691528657672541No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT72790.13667119733845096No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC70800.1329347543833264No Hit
GAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGCATCCTG70720.1327845456213113No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC69670.13081305561986367No Hit
AGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATG68480.12857870028488969No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC68470.1285599241896378No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCT68440.12850359590388216No Hit
GCTTTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGG67930.1275460150460362No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT67900.12748968676028052No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC67520.12677619514070898No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC67490.12671986685495334No Hit
GAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAGCC66950.1257059577113517No Hit
GTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACT65910.12375324380515595No Hit
GATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCCT65200.12242014104227232No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA64210.12056130761233597No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG63140.11855226542038456No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA62630.11759468456253856No Hit
GGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCT62460.11727549094325658No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC60640.11385824160741399No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT60390.11338883922611695No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG59170.11109815560538731No Hit
GGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGT58870.11053487274783085No Hit
GCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGG57720.10837562179386438No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT57340.10766213017429287No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT55870.10490204417226616No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT55820.10480816369600676No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC55640.10447019398147288No Hit
GGCAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGCATCC55620.10443264179096912No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC54530.10238604740851395No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC54360.10206685378923194No Hit
GTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTC53660.1007525271216002No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC53510.10047088569282196No Hit
GGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAA53370.10020802035929562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG251.2936471E-7117.634718
GGTTAGA550.0117.628091
GAAGCTA20250.0113.5619351
GGATCTG70600.0113.462791
GAGAACT42300.0113.456881
GATTCCT39350.0109.407571
GACTGCA4700.0108.868551
GATCACT24450.0108.7278061
GGATCCT49700.0108.161041
GGCTTTC24850.0108.161041
GACATTA19850.0107.2578661
GCTTTAC7700.0106.934631
GATGTTC203700.0106.6852651
GTTACTA2650.0106.531111
GAGAGAC59800.0106.31771
GCTTCAA10050.0105.9238051
GACTTCG4500.0105.865281
GGTTCAC13300.0105.68841
GATCCTT22850.0105.530671
GGCTTAC4450.0104.4114461
Produced by FastQC (version 0.11.5)