FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044897
Sequences flagged as poor quality0
Sequence length111-123
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT6962078.654020057683772No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTA2078922.5841474415396495No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG1775882.207461450407631No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1708902.1242037032916645No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC1657022.059715618484612No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1496331.8599740928939175No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTC1344171.6708355619717692No Hit
GAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGG789090.9808578034000932No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT766290.9525168563376263No Hit
GACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCCTGGA762710.9480668304392212No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT653740.8126145058165443No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT648000.805479548091169No Hit
ACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATG549140.6825941960475068No Hit
GATCCTGTCCTGACCCTACCATGGGCACCAGTCTCCTATGCTGGGTGGTC456430.5673534415667472No Hit
GTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACC349510.43444931613170434No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT342650.42592217153308487No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC340920.42377174002352047No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT338410.4206517497986612No Hit
GTCTTCCCCAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCC337200.4191476907659601No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA335640.4172085733353702No Hit
GGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCA302600.37613906057467233No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC299430.3721986745137943No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG277540.34498887928583793No Hit
GACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCA272830.3391342362742494No Hit
GCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTAC272150.3382889799583512No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC269430.33490795469475865No Hit
GGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGG263000.3269153104135454No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT254290.3160885714260854No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT251280.31234706920424216No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG247850.3080834969049324No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC233370.29008450947227793No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC232630.2891646717167417No Hit
GGCTGCTGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTG224880.2795312357634908No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC215740.26816999645862466No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG214690.2668648212649584No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG214270.2663427511874919No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG206840.2571070829123107No Hit
GGCTGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACC202940.25225928933583613No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC188170.23389982494493095No Hit
GACACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCCTG182870.2273117977769013No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC179810.2235081443553597No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG173800.2160375701516129No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT173340.21546577911438763No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT172560.21449622039909272No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT170100.2114383813739318No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG169180.2102947992994814No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT167890.20869129834726285No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC155250.19297947506350918No Hit
GCTTCCCCAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCC151950.1888774958834153No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT149430.1857450754186163No Hit
ACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCCTGGAG144160.1791943389704057No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA142710.17739195417915232No Hit
GACACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACC141840.1763105233044003No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG136870.17013269405438006No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC136730.16995867069522455No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT132880.16517302831844832No Hit
CTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACC131930.16399215552417887No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC129190.16058626978070695No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC128340.15952969938583428No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT128330.1595172691458946No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG128040.15915679218764392No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC126050.1566831744396479No Hit
GGACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCA125340.15580062740393072No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC125270.15571361572435297No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG124560.1548310686886358No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG123510.15352589349496953No Hit
GACCCTACCATGGGCACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCT123140.15306597461720142No Hit
GCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCA122740.1525687650196143No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC117280.1457818540125498No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT116280.14453883001858198No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG115380.1434201084240109No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG110070.1368196510160416No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC108900.13536531294309923No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT106750.1326928113560683No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG106300.13213345055878278No Hit
GATCCTCTGCTGCTGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCC105550.1312011825633069No Hit
GGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTGCTGG105040.1305672403263833No Hit
GTCAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT104960.13046779840686587No Hit
GAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGCCAT104920.13041807744710715No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT104910.13040564720716746No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC101450.1261047841880387No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT101390.12603020274840065No Hit
CAGTGTAGGCAGAGGAGGAACTGTATCACCACAGAAACTTCTGCCTTCAC101260.1258686096291848No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC98620.12258702628510967No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC95860.11915628006175841No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT94930.1180002677473683No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG93580.11632218535551171No Hit
GATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTCT90800.11286657865228107No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC87910.10927423930971397No Hit
GCTATTTCCACAGATCACACAGGTGCTGGAGTCTCCCAGTCTCCCAGGTA87590.10887647163164427No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG87070.10823009915478098No Hit
GGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTC86040.10694978444099408No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC85360.10610452812509594No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT85180.10588078380618174No Hit
GCTTCACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCC84850.10547058588817233No Hit
GCTCTGCTGCTGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGT84570.10512253916986135No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT83080.10327043341884924No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG81490.10129402526844035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)