FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044897
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC102275212.713052758786098No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG2179742.7094691206114883No Hit
GGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG1475051.8335225423022818No Hit
CGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCAACC1031011.2815701680207963No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC950201.181121399068254No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG901701.1208347353608132No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC650410.8084752359166314No Hit
GTTCTCTGCAGAGAGGCCTGAGTGATCCATCTCCACTCTGACGATCCAGC523760.6510462470806028No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT499100.6203932753893555No Hit
CAGTCTCCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC415930.5170109698110491No Hit
GTGCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC395120.4911436404965781No Hit
GTTCTCTGCAGAGAGGACTGAGGGATCCATCTCCACTCTGACGATCCAGC324970.4039455073197333No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC298960.37161445323662934No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA251880.3130928836006229No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC249560.31020906793461744No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA245600.30528669291850474No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT241610.300327027182573No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC231020.28716340308645344No Hit
GTTCTCTGCAGAGAGGCCTGAGGGAGCCATCTCCACTCTGACGATCCAGC223730.27810175817042776No Hit
CCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTG221800.27570272186206984No Hit
AGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCAACC218790.2719612196402266No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT207630.25808907186754537No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC196090.24374457497715632No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT188050.23375066206565479No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC174810.21729302438552042No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG167060.20765958843226956No Hit
GTTCTCTGCAGAGAGGCCGGAGGGATCCATCTCCACTCTGACGATCCAGC162610.2021281316591126No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC158290.1967582680051715No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCAGCTCCACTCTGACGATCCAGC157470.19573898833011782No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG149230.18549647061982272No Hit
GCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC148220.1842410163859152No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC141370.1757263020272354No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA140590.17475674331194047No Hit
GTTCGCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC140030.17406064987531847No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC137670.17112711324955435No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGAGCCAGC136590.16978464733606907No Hit
CGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG133080.1654216331172419No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC132570.1647876908803183No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT132520.1647255396806199No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATGCAGC121820.15142518294516388No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG120860.15023187991095474No Hit
GATCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC120190.14939905383499627No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG119910.14905100711668529No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC119590.14865323943861555No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT119130.14808144840139034No Hit
GGTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC117530.1460926100110418No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT116680.1450360396161691No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA115780.14391731802159805No Hit
GTTCTCTTCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC111560.1386717567670537No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC111210.13823669836916494No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA97700.12144344421065925No Hit
GTTCTCTGCAGAGAGGCATGAGGGATCCATCTCCACTCTGACGATCCAGC96930.12048631573530401No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC96450.11988966421819942No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG95170.11829859350592059No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG93930.11675724375340045No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC90660.11269255529312557No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA89440.11117606602048478No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA88630.11016921658537082No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG88540.11005734442591372No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG85500.10627855148425144No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT85250.10596779548575949No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC85220.10593050476594044No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG85150.10584349308636269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)