FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4945295
Sequences flagged as poor quality0
Sequence length109-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC2895545.85514109876155No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1945393.933819923786144No Hit
GAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTG1644793.3259694315505954No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1302312.633432383710173No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG1054502.1323298205668215No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC919051.8584331167301447No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG885541.7906717395018903No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT802451.6226534514118978No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG726741.4695584388797838No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC680111.3752667939930783No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC481760.974178486824345No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC442320.8944259139242452No Hit
GGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCT426200.8618292740877945No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA419690.8486652464615357No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT410410.829899935190924No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT407460.823934669215891No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT392540.7937645782506402No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG375540.7593884692419764No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC351440.7106552794120472No Hit
GAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTC333920.6752276658925302No Hit
GACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTA319630.6463315130846593No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT280030.5662554003350659No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT273240.5525251779721937No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT270340.5466610182001276No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT249400.5043177403976912No Hit
GAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTC240630.4865837123973393No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC236540.47831322499466666No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT233780.47273216259090717No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT219100.4430473813998963No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT217880.4405803900475098No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT183040.37012958782034233No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC178600.3611513569969031No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG178280.3605042772979165No Hit
AGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCT177470.3588663568098566No Hit
AGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCT169640.343033125425278No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG164410.3324574165949655No Hit
GACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCCTGGA164360.33235631039199887No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT153700.31080046791950733No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG146110.2954525463091686No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC138370.2798013060899299No Hit
GACTGAGAGCCCAACTTCAGTCTGCCCACAGCAGGGCTGGGAGACACAAG136260.27553462432473697No Hit
GAACTTCAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTG135500.27399781003964374No Hit
GGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTT133080.26910426981605745No Hit
GACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCA132730.2683965263952909No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA127390.2575983839184518No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG126590.2559806846709852No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT124650.2520577639958789No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG121170.24502077226939953No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC121110.24489944482583953No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT121090.24485900234465285No Hit
GACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTATG120340.24334240930015297No Hit
GATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTGGGTCCTGCC119070.24077431174479988No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT112430.22734740799082764No Hit
GGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGC110880.22421311569886124No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG110410.22326271739097464No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG107210.21679192040110853No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT106470.21529554859720199No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC106380.21511355743186203No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC102000.20625665405198274No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC98670.1995229809344033No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC98010.19818837905524342No Hit
GCAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCT97480.19711665330379682No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC97040.19622691871769024No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT95410.1929308565009772No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG93200.1884619623298509No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC90410.18282023620431137No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC90060.18211249278354477No Hit
GGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCC89060.1800903687242116No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC87980.17790647474013177No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG87330.1765920941015652No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC81570.1649446595198062No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG79650.1610621813258865No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT78080.15788744655273346No Hit
GACAGGGCCAAGAGACAGATTTGCTTTCCTTTTTCTCATGCTTGTAAGCT76270.1542274020053404No Hit
GCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTG75720.1531152337727072No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT75690.1530545700509272No Hit
ACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTAT74980.15161886196880064No Hit
GACACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC74780.15121443715693403No Hit
ACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGG72400.1464017818957211No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT71860.14530983490368118No Hit
GACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCTCTTCTTCTGTG70810.14318660464138136No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC70500.14255974618298808No Hit
GAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCA70400.14235753377705473No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA70320.1421957638523081No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT67510.1365135952455819No Hit
GGCTTCCCTCAACATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCAT66710.13489589599811538No Hit
GAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTG66440.13434992250209543No Hit
GAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCC65850.13315686930708887No Hit
GAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGGC64880.1311954089695357No Hit
AAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCTCTTCTTCT63040.12747470070036268No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC62160.1256952315281495No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG61670.12470439073907623No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT58450.11819315126802345No Hit
GAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGG58450.11819315126802345No Hit
AGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCCTCTGCT58320.11793027514031013No Hit
GACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTATG57700.11667655822352356No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT57370.11600925728394362No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC57050.11536217758495702No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC55810.11285474375138389No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC55230.11168191179697065No Hit
GGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGAC53620.10842629206144426No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC53070.10731412382881103No Hit
GATGGGAGCTCAAAATGCCCCTCCTTTCCTCCACAGGACCAGATGCCTGA52830.10682881405457106No Hit
GCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCC52000.10515045108532453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC4750.0102.228561
GAGAAAC113000.0102.0077741
GAGAACT108050.0100.314351
GAAATCC17650.098.67884116
GCTTTTC100350.098.160961
GAGAGCT363950.098.127961
GGCTTCC9400.096.551351
GATCTCA50300.096.422981
GCTTCAA58300.095.883641
GATTCCT139400.095.5446241
AGTGGTA300500.095.3992542
GACTACT16850.095.374321
GAATGCG7700.095.345361
GAGTCAG47900.095.099291
CCAAGGA382150.094.395836116
GGACGTT3500.094.144161
GGATCTG22600.093.617971
GACTACG18600.093.549221
GAAATAG3000.092.492511
GAGATGC82650.092.464531
Produced by FastQC (version 0.11.5)