FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4945295
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG1740293.5190822792169127No Hit
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC1472032.9766272790601978No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT1366062.7623427924926625No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG1236912.501185470229784No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC1214832.456536970999708No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1003522.029241936022017No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT911731.843631168615826No Hit
GGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG630321.2745852370788802No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA593681.2004946115449129No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT455210.9204910930490497No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA454830.9197226859065031No Hit
AAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTGCTCCCTCCCAAA433410.8764087885555867No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG414790.8387568385708031No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC399070.8069690483580858No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG280200.5665991614251525No Hit
CTTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT253650.512911767649857No Hit
GGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC240480.4862803937884393No Hit
CTACGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGT219400.4436540186176962No Hit
CCAATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGT205540.41562737915533854No Hit
GTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGT202710.4099047680674257No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA201270.4069929094219859No Hit
CGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC201250.40695246694079934No Hit
TCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC197320.3990055193876199No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG184650.37338520755586874No Hit
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC183150.370352021466869No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC174820.35350772805262376No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC170800.34537878933410443No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG168240.3402021517422115No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC157130.3177363534430201No Hit
CGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG147960.29919347581893496No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC140060.283218695750203No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC134930.27284519932582385No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTCCACACCCTGC134170.2713083850407306No Hit
CCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC133630.2702164380486907No Hit
CCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC120610.24388838279617292No Hit
GCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC104460.21123107923794235No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC99980.20217196345212973No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC99110.20041271552050988No Hit
CAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCC85780.1734578018095988No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA84560.17099081045721234No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC84200.17026284579585244No Hit
CCCTGACTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC84120.17010107587110576No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA82930.1676947482404993No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG81460.16472222587327953No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA81090.16397403997132629No Hit
CCCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACACCCTGC80780.163347181512933No Hit
CGTGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG79860.1614868273783465No Hit
CCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA77200.1561079773805203No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT75760.15319611873508052No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG74600.15085045482625406No Hit
CGCGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG72340.1462804544521611No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC69660.1408611619731482No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC68660.13883903791381505No Hit
CAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC65670.13279288697640887No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG65230.1319031523903023No Hit
AAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC64280.12998213453393578No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT63680.12876886009833588No Hit
CTCAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCA62580.12654452363306942No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG61400.12415841724305629No Hit
GGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC59320.1199523991996433No Hit
GTTCTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGC58270.11782916893734348No Hit
TCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC56230.11370403585630381No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC54680.11056974356433741No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA54290.10978111518119749No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGACTTTCCCCCTGATCCTGG52090.10533244225066452No Hit
CGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC51780.10470558379227124No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT51000.10312832702599137No Hit
GGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG50820.1027643446953114No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA50250.1016117339814915No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC49900.1009039905607249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)