FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216072
Sequences flagged as poor quality0
Sequence length110-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2642545.0661493936433395No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC1126402.1594793936893506No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1093812.0969994279220074No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT689571.3220101256271002No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT615201.179431572263573No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT611141.1716479373750976No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT565631.0843983748690584No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC486870.933403526638436No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT449060.8609160303001953No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG413600.7929338398703085No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT406990.7802614687834063No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC382420.7331570576479772No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT379430.7274247748113906No Hit
GATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTGGGTCCTGCC342370.6563751420609225No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA313650.6013145524064851No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT302410.5797657701044003No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT300640.5763724120372572No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG251450.48206773219388077No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG250920.4810516419251881No Hit
GACAGGGCCAAGAGACAGATTTGCTTTCCTTTTTCTCATGCTTGTAAGCT239580.45931114447806703No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT226580.43438817562334264No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC224800.43097564604169575No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC197180.3780239229826582No Hit
GATCCTGCCCTGACCCTGCCATGGGTACCAGGCTCCTCTGCTGGGTGGCC187310.3591016381675713No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT183130.3510879451050522No Hit
GACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGTGTG180570.3461800373921219No Hit
GGCTCCTGCTCACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTG174250.33406364022582513No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG173240.33212731726095807No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG173090.33183974454340354No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC170940.3277178689251222No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC168590.32321256301676815No Hit
GGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCATGGGTACCAGGC167200.320547722500763No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT166720.31962748980458855No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC163310.31309000335884934No Hit
GGACAGAGGTGGAGAGAGCCACACAGATAGCCAGCTGCCTGTGCTGCCTG161430.30948575863216615No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC160670.30802872352988997No Hit
GATGTGCCTCAGACTTCTCTGCTGTGTGGCCATTTCTTTCTGGGGAGCCA156130.29932485594524No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG155520.29815539356051834No Hit
GCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTG151160.28979661323693384No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG149910.2874001739239796No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC148760.28519544975606165No Hit
GCACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTG147100.2820129783484584No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC144190.27643406762790085No Hit
GGAGCCAACCATGTGCCTCAGACTTCTCTGCTGTGTGGCCATTTCTTTCT143590.2752837767576828No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG140880.27008829632719794No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT139470.2673851127821855No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC138440.2654104467883112No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT137400.26341660927993327No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA132550.2541184247456707No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC131230.251587784831191No Hit
GACATCTTACAGGGCCAAGAGACAGATTTGCTTTCCTTTTTCTCATGCTT126180.24190617000685574No Hit
GAGTTCCAAGACTCTCTCCGGTGGCTGCAGTATCAAGCTCCCTCCTGCGC121150.23226289821152776No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC120680.23136183702985696No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC120390.23080586310925155No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT117820.2258787838818176No Hit
GGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGTGTGGC117170.22463263543908135No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC115700.22181442280704713No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT114850.22018484407423825No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC113670.21792260536280939No Hit
GGACCCTGCCATGGGTACCAGGCTCCTCTGCTGGGTGGCCTTCTGTCTCC109900.21069494439493933No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT108900.20877779294457594No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG105450.20216362044082214No Hit
GACACAGATAGCCAGCTGCCTGTGCTGCCTGCTCTTCCCCTAATTCTGCC105420.2021061058973112No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG103460.19834848905459895No Hit
GCATCCTGCCCTGACCCTGCCATGGGTACCAGGCTCCTCTGCTGGGTGGC100740.19313383710961043No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA100320.1923286335004578No Hit
AGTTCTTCAAGAAACGGAGATGCACAAGAAGCGATTCTCATCTCAATGCC100050.1918110026088597No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG99960.191638458978327No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT96460.18492842890205505No Hit
AATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCCT95970.18398902469137696No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC95250.1826086756471153No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC94410.18099826842881003No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC93780.1797904630150811No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC92510.17735568067311955No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG92190.17674219220900325No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC88480.16962956032815496No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT87710.16815335371137516No Hit
GGCTTTCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATT86570.16596780105796086No Hit
GACTGTAGGAACTTCTCTGTAAGGACAGCAACATCCCACTTCCTCTGCTC85970.16481751018774282No Hit
GCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGTGTGGCCATTT85320.16357136174500658No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT85160.16326461751294843No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT83860.160772320627476No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC80940.15517423839241484No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG80100.15356383117410957No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA79780.15295034270999328No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG77510.14859840891766832No Hit
GAGAGGTGGAGAGAGCCACACAGATAGCCAGCTGCCTGTGCTGCCTGCTC77090.1477932053085157No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG76080.14585688234364863No Hit
GACAGAGAAGCCGCCGACAGAGGTGGAGAGAGCCACACAGATAGCCAGCT73960.1417925212688782No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT73450.14081477402919285No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC71910.1378623607956332No Hit
GCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT71310.13671206992541515No Hit
GCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAG71150.136405325693357No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG70360.1348907760475699No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC68410.13115233071936122No Hit
GGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT68050.13046215619723042No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG67540.12948440895754507No Hit
GGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCCTGG65950.12643613815146723No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT65180.12495993153468739No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG64420.1235028964324112No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT64170.12302360856982035No Hit
GGCAACGTCAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCT64120.12292775099730219No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC62050.11895924749504992No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGGGCACCAGTC59900.11483737187676857No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT58110.11140567078061807No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG57190.10964189144628371No Hit
ACTCGGCCGTGTATCTCTGTGCCAGCAGCTCACGGGACAGGGTGGAGACC56550.10841491451805113No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT55830.10703456547378946No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT54960.1053666437119733No Hit
GGCTTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGT54350.10419718132725161No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT53950.10343032074710626No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT53230.10204997170284459No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG53000.10160902686926102No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG52760.1011489105211738No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG52720.10107222446315925No Hit
GACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTG52640.10091885234713018No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC52570.10078465174560473No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT52430.10051625054255386No Hit
GGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGA52330.10032453539751751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)