FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216072
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGT1635063.134657650431206No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1123282.153497881164217No Hit
TCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC711961.3649351466007371No Hit
CTCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAATCCTGTCCTCAG533191.0222059818192695No Hit
CCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC473400.9075794966020407No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC411120.7881793042734073No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC406520.7793604076017355No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC392660.7527886884996986No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA389650.7470180626341048No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC365450.7006229975353101No Hit
CGCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGA356630.6837137217431049No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG348650.6684148531692047No Hit
ATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAATCCTGTCCTCAG344230.6599410437585984No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC328760.6302827108214764No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG297090.569566524388467No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA294890.5653487911976676No Hit
GGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGA286350.548976317811564No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT250470.4801889237725246No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT246450.4724819749420637No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT245070.46983630594056214No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG239160.4585059408689144No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC229520.4400246008874111No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG228510.438088277922544No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC227010.4352125507469989No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA224830.43103316058520663No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG211000.40451895602668064No Hit
CAATGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGA198020.3796343302009635No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT187860.3601560714652712No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT183500.3517972911416867No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG183180.3511838026775704No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG176720.33879900430822274No Hit
CTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG175060.33561653290061944No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG158430.3037343042810759No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT154460.2961232130231331No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT145000.2779869603026952No Hit
CCTGGTGGAAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC144330.27670246883095173No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA137340.26330158019291144No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG134530.25791438461739025No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC118050.22631972871540118No Hit
CTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG117430.22513109481617585No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG111400.21357067157048445No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG111090.21297635462087178No Hit
GCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC110730.21228618009874098No Hit
AGCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGA109680.21027317107585938No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC103050.19756245695994995No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC102700.19689145395232274No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG95660.1833947077417643No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG95270.18264701867612257No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG94170.1805381520807228No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC94160.18051898056621918No Hit
CCACTGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGT91760.17591781708534698No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA91630.17566858739679975No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC85780.16445325141217376No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC85550.16401230657859017No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT84180.16138580909159228No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC83680.16042723336641057No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG83310.15971788732977613No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC82170.15753233467636182No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC81240.15574938382752385No Hit
CAAAGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGA80290.1539280899496786No Hit
CGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG80100.15356383117410957No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG77470.1485217228596538No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG75490.14472576298793421No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT71300.1366928984109115No Hit
CCTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG69380.13301196762621376No Hit
GGAGTCTCCCAGTCTCCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGA66610.1277014581087071No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC64810.12425058549805294No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG64290.12325366674386397No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA62680.12016705290877887No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG61740.11836493054543724No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC60020.11506743005081219No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA60010.11504825853630854No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT58910.1129393919409088No Hit
GGTCATGCTTTGTCTCCTGGGAGCAGGTTCAGTGGCTGCTGGAGTCATCC57390.1100253217363564No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC56770.10883668783713109No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG54660.10479149827686428No Hit
GAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAAGCTTG54140.10379457952267529No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTCCACACCCTGC54000.10352617831962442No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG53950.10343032074710626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)