FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5503211
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1835823.3359069823054215No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT1341632.437903980058188No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT548590.9968543819235716No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT434060.7887395195277811No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG399030.7250857726516392No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC346840.6302502302746524No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT306300.5565841469643814No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG267620.4862979086209851No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC251710.4573875143075561No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT240810.43758089595328986No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG218210.39651396248481113No Hit
CGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA173130.3145981500618457No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT162630.29551838008755255No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG158960.28884954620129955No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA143890.261465533485814No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT143350.26048428817285035No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC141670.2574315249769634No Hit
GTTCTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGC129020.234444944960315No Hit
GTTCTCTGCAGAGAGGCCTAAGTGATCTTTCTCCACCTTGGAGATCCAGC121010.2198898061513542No Hit
CAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCA119290.21676435811747No Hit
GTGCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC116840.21231241179013488No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA116700.21205801485714432No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA113640.2064976247503503No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA110750.20124614520504483No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC108670.19746653362918484No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG104540.18996182410596288No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT103970.18892606516450122No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT102810.18681820486257933No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA95750.17398933095605457No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC92990.16897407713424034No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA90370.16421322024541674No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC87640.1592524800521005No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC87640.1592524800521005No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC86910.15792598175864964No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT84750.15400100050679502No Hit
CTACACTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT78490.14262582336021642No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC77290.1404452782202972No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC72320.13141418709913177No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA71200.12937901163520715No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA69620.1265079605343135No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG65040.11818554658362182No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG64300.11684087708067163No Hit
CTACAATGTCTCCAGAGCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT64250.116750021033175No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA63330.11507826975923692No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA62670.11387896993228135No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA61970.11260698526732849No Hit
CTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC58800.10684671185604187No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA58760.10677402701804455No Hit
CCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTG56500.1026673336711967No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA56270.10224939585271219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC961700.0143.72809145
CGTGCCC335250.0142.33469145
AGTTGTC341800.0142.30083145
CGTACTG178550.0141.9087145
CGTGCTA155200.0141.63129145
GGTCACC917950.0136.8204145
CCGATTC57100.0136.106981
TTGACCC75000.0135.2316145
CGTGCGC87850.0133.27617145
GGTCACA94850.0132.68875145
GGTGCTC169550.0132.55179145
CTATAGT136200.0132.113251
AGTGCTC526000.0131.4461145
CGTTGTC170100.0130.92982145
TATAGTG132300.0130.363592
GGGTTGC97900.0130.256949
ATAGTGT125750.0129.83213
CGTTGCC67600.0129.55135145
ACAATGT313000.0128.781113
GGTGCCC59950.0128.18533145
Produced by FastQC (version 0.11.5)