FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6220149
Sequences flagged as poor quality0
Sequence length106-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1533792.465841252355852No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA1207101.9406287534269677No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC1186721.9078642649878643No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1159201.863620951845366No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1111741.787320528816914No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1033421.6614071463561402No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG774341.244889792832937No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT614340.987661228050968No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT599810.9643016590117053No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT585120.9406848614076608No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT571650.9190294316100788No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT498270.8010579810869483No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA416960.6703376398218114No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC387510.6229915071166302No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC368860.5930083025342319No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC343270.5518678089544158No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC342980.5514015821807484No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT311500.500791862059896No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC280900.45159689904534445No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT274930.44199905822191715No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG257830.4145077553608443No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG254660.4094114144211015No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC250550.40280385566326465No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG237000.3810198115832916No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG231250.37177566003643964No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG230860.3711486654097836No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT224900.36156690137165526No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC220790.3549593426138184No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG220670.35476642119023194No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT216710.3484000142118782No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC215130.34585988213465624No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA208760.3356189698992741No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC201430.3238346862752002No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG196790.3163750578965231No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT195760.31471914901073916No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT187570.30155226185096207No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC186690.3001375047446613No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC184600.2967774566171968No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT183880.29561992807567794No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC179940.28928567466792193No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG177730.285732705116871No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC169850.27306419830135903No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT169790.2729677375895658No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT166360.26745340023205233No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT165390.26589395205806166No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC162120.26063684326533015No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA159130.2558298844609671No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC155160.24944740069731447No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT153450.2466982704112072No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG148420.23861164740587404No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT147170.2366020492435149No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG146850.23608759211395097No Hit
CCAACGTCCTGACTTTCGGGGCCGGCAGCAGGCTGACCGTGCTGGAGGAC143870.23129671009488678No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC142110.22846719588228512No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA139300.22394961921330178No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA138760.22308147280716267No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC138610.22284032102767953No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC137350.22081464608002155No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG136760.21986611574738804No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA136310.21914266040893876No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA133330.2143517783898746No Hit
GGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGG132310.21271194628938953No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC131300.21108819097420337No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG130030.20904643924124647No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC126890.20399832865740034No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG126030.20261572512169723No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC124820.20067043410053362No Hit
GCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCC123180.19803384131151844No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT122330.1966673145611142No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT111960.17999568820618284No Hit
GCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAA110080.17697325256999472No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT109010.1752530365430153No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG107950.17354889730133474No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT107190.17232706161862038No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC107070.1721341401950339No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC102980.16555873500779483No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA101440.1630829100717684No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC98860.1589350994646591No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG98340.15809910662911772No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT96560.1552374388459183No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG96200.1546586745751589No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC96190.15464259778986No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG95680.1538226817396175No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG95060.15282592105108736No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG94970.1526812299833975No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC94670.1521989264244313No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC94510.15194169785964934No Hit
GCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCA92920.14938548899712853No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC92710.1490478765058522No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC91580.14723119976707955No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC87270.14030210530326526No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC86740.13945003568242498No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG84970.13660444468452446No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG84240.1354308393577067No Hit
GTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC83090.13358200904833628No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT81390.13084895554752787No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTACCAATCTG79730.12818020918791495No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA78880.12681368243751073No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT77940.12530246461941666No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC76950.12371086287482824No Hit
AGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCT76630.1231964057452643No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT76250.12258548790390714No Hit
GGTTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCC75850.1219424164919522No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC75500.12137972900649165No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC74730.12014181653847841No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT73910.11882352014397082No Hit
GACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCA73700.11848590765269448No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG71650.11519016666642551No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC71100.11430594347498749No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC70970.11409694526610215No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT69070.11104235605931627No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG67610.1086951454056808No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC67480.10848614719679545No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG65880.10591386154897575No Hit
GGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCATACC65820.10581740083718252No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG65410.10515825263992871No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC64900.1043383365896862No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA64430.10358272768063917No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT64140.10311650090697184No Hit
GAGCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCT62480.10044775454735892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTG17000.0102.1451641
GGATCTG61450.0100.693461
GACTGTT5750.098.65151
GCACTAC2250.097.75671
GAGAGCT153900.097.2978441
GCTTTTC51150.094.8295751
GCCCGAC13450.093.816591
GACAGAC217700.093.722991
CATTGAG18550.092.980817
AGTCATT20300.092.6678854
ATTGAGG17550.092.34338
AATGCTT81150.091.44114
GACTGCA7500.091.068081
GAGAACT14850.090.818631
AGAATGC83150.089.868182
TGCTTAC82600.089.6957556
GGAGAGT31700.089.6535951
GAGTGAT32650.089.349641
GATGTTC145800.089.1656651
ATCTGAT173300.089.06888116
Produced by FastQC (version 0.11.5)