FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4223063
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1876624.443741426542772No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT930442.20323495055603No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT396470.9388209458395482No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT236250.5594280738885496No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG188780.4470215102166366No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT177990.42147133490549393No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT168400.3987626990172773No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC156450.37046570226397285No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG134990.31964950558398014No Hit
GTTCTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGC134890.31941271063206966No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC129610.3069099371711954No Hit
CAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCA128550.30439991068094413No Hit
GTTCTCTGCAGAGAGGCCTAAGTGATCTTTCTCCACCTTGGAGATCCAGC128310.30383160279635896No Hit
GTGCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC124020.2936730993593986No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA119610.2832304419801457No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA116830.27664754231703387No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC112270.2658496925099152No Hit
CGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA109390.2590299978948929No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG104800.24816110960220106No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT99870.23648711847301354No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG99490.23558729765575365No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA96480.2284597696032477No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA81810.19372195015797775No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT78500.18588403724974029No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG78070.18486581895652515No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT76960.18223739499031866No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA73270.1734996612648213No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC72970.17278927640908978No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA69110.16364899126534463No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT68930.16322276035190572No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT65170.15431927016007102No Hit
CTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC64030.15161980770829134No Hit
CTACACTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT59310.1404430859781159No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC52700.12479093965683202No Hit
GTTCTCTGCAGAGAGGCCTAAGGCATCTTTCTCCACCTTGGAGATCCAGC51200.12123901537817457No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA49940.1182553989841023No Hit
CGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCTTGG48230.1142062053064328No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC48100.11389837186894915No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA47760.11309326903245345No Hit
CTACAATGTCTCCAGAGCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT47140.11162514033060839No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC44340.10499488167711446No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC44300.10490016369635025No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG43030.10189286780708694No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA42690.10108776497059124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC794350.0143.80632145
AGTTGTC216050.0141.63722145
CGTGCCC280200.0141.57751145
CGTGCTA126550.0141.15456145
CGTACTG140300.0141.06566145
CCGATTC45850.0136.14471
GGTCACA82100.0135.8976145
GGTCACC793250.0135.69823145
TTGACCC36550.0133.6869145
GGTGCTC139850.0133.43274145
CGTGCGC76750.0132.71326145
CTATAGT101400.0132.201341
ATAGTGT91850.0132.054643
ACAATGT220650.0131.626683
GGGTTGC48200.0131.302589
TATAGTG97100.0131.261262
CGTTGTC100500.0129.77213145
AGTGCTC381150.0129.60522145
GGTGCCC45500.0129.0595145
TATCGTG12750.0128.509482
Produced by FastQC (version 0.11.5)