FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7822152
Sequences flagged as poor quality0
Sequence length111-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG4764126.090548994701202No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1653152.1134209613927215No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1159531.4823670007946663No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT987381.26228690007558No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT918041.173641217915479No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT828791.0595421822536815No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC811391.0372976643767597No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT753070.9627401768720423No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC617660.7896292478080201No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT493160.6304658871369414No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC483840.6185510074465441No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG462040.5906814390720099No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG439780.5622237972363615No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG389950.4985201003509009No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG387130.4949149543501584No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG348100.44501819959520095No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC335310.42866720053509566No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC322990.41291705914178095No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT318640.40735592967255047No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT316610.4047607359202429No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT258810.33086802711069796No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT242930.31056670849658763No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT239050.3056064366941476No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC236890.302845048268047No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG234760.3001220124589755No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG233590.29862626039483764No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG232810.29762909235207907No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC222720.2847298288245997No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC220660.28209628245526297No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC219900.28112468282385716No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG218150.2788874468304886No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT206830.264415726004813No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT203280.2598773329896939No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT201560.25767844961335445No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC199360.25486592436454825No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA192670.24631329076704211No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG190020.24292547626279826No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG189810.24265700794359404No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC187690.23994675634019896No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT187260.23939703549611413No Hit
CAACATCTGGGTCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAA184930.2364183155735148No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC184580.2359708683748411No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC184290.23560012641022574No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC182440.23323504836009323No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG182160.23287709060115427No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT175930.22491253046476212No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC174520.22310995746439086No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC173830.22222784727271983No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC166910.21338117694465666No Hit
GATCCTGCCCTGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCC162900.20825471046842353No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT161610.20660554793616898No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC157520.20137680781452472No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA151370.1935145213235437No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG150560.19247900066375598No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA149590.19123893271314596No Hit
GCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT148840.19028011728741656No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG141510.18090929452662133No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG141500.18089651032094492No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT139040.17775159572455254No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT137300.17552714393686034No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC134640.1721265452269401No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG132860.16985095661654234No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT129630.16572165818306778No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC128260.1639702220054021No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC128020.1636634010691687No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG121780.15568605672710017No Hit
AATGTGAACAACATCTGGGTCATAACGCTATGTATTGGTACAAGCAAAGT120250.1537300732586122No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA118290.1512243689460394No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT118220.15113487950630466No Hit
GACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCA114360.1462001761152174No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC112920.14435925049781698No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC112030.14322145619261809No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC111960.14313196675288337No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC109310.1397441522486395No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA108810.1391049419648199No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCCCCTGCCATGAGCATCGG106460.13610065363086782No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA106400.13602394839680945No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCCTGCCATGAGC104980.1342085911907618No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT104770.1339401228715576No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTC101860.13021991901972757No Hit
GACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATCGG101790.13013042957999282No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC99730.1274968832106561No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG98960.12651249937357392No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT96250.12304797963527173No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT95400.12196132215277843No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC95270.12179512747898533No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG93450.11946840204588201No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT93270.11923828634370695No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG93050.11895703381882633No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA90380.1155436509032297No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT89550.11448256183208919No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG88560.11321692547012636No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT88330.11292288873956938No Hit
GACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCT88270.11284618350551102No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCCTGCCATG88100.11262885200901236No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCCT87540.11191293649113442No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC86720.11086463162567027No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC86090.11005922666805759No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC85710.1095734268523547No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC85040.10871688507203645No Hit
CAGACACCAAGACACCTGGTCATGGGAATGACAAATAAGAAGTCTTTGAA83890.10724670141925138No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC83430.10665862795813735No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT82600.10559753888699684No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCCTGCCATGAGCATC82430.10538020739049817No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCC82170.10504781804291198No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC81720.10447252878747434No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG81290.10392280794338948No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT81030.10359041859580331No Hit
GGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCC80730.10320689242551155No Hit
AATGACAAATAAGAAGTCTTTGAAATGTGAACAACATCTGGGTCATAACG80320.10268273999277948No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC78880.10084181437537904No Hit
GGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT78620.10050942502779286No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC78580.1004582882050873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)