FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031307
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC1167801.4540597190469744No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC925611.1525023262091711No Hit
CGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC848641.0566648740983255No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC547360.681532906163343No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT513390.6392359300920759No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG480760.5986074246694841No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG464370.5781997874069563No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG434160.5405844901707779No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC374080.4657772389973388No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA365540.45514385143040853No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG361560.4501882445783731No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG345540.4302413044352557No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC343910.4282117468551507No Hit
CGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC338020.42087794676507817No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC314580.39169216168675913No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC300820.3745592093540939No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA298380.3715210986206853No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG297810.3708113760313234No Hit
AGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC290430.361622336190112No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA284820.35463717175797166No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC280940.349806077640912No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC279530.3480504480777537No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT264200.32896264580596907No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA249420.31055966357655107No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC246510.3069363429887564No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA229690.2859933009658328No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG216530.26960742504302226No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG207840.25878726837362837No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA200970.25023324348079334No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA192590.23979907628982433No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT183710.22874234542397645No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG183570.22856802759501038No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG183400.2283563559455516No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC182820.22763418208269215No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC182470.227198387510277No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT179350.22331359017903316No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT177800.22138364278690875No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC166500.20731370373464741No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC163030.2029931118309884No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC161060.20054021095196584No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC159500.19859781228634393No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC159110.19811221261993844No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA158970.19793789479097237No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA156050.19430212292968005No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT150690.1876282403349791No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG150610.1875286301469985No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG148910.1854119136524105No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG146590.18252321820097275No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA144080.17939794855308108No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG141740.1764843505546482No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG141480.1761606174437112No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA140880.17541354103385662No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC139970.17428047514557718No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT136900.1704579341818212No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT126980.15810627087222542No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA123580.15387283788304942No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG120420.14993823545781526No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC115970.14439741875139378No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA113050.14076164689010146No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC112970.14066203670212085No Hit
CCCTGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGA111390.13869473548950376No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC107990.13446130250032778No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC107390.1337142260904732No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT105490.13134848412593367No Hit
GCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG105340.13116171502347002No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG104490.13010335677617604No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA104100.12961775710977055No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC104000.12949324437479479No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC103930.12940608546031176No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA101220.12603179034246856No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA100320.12491117572768666No Hit
GGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC98910.1231555461645284No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC98870.12310574107053808No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACAGAGC98700.12289406942107928No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA97750.12171119843880952No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA95230.11857347751742027No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC91140.1134809066569115No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC88490.11018131918005376No Hit
TGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGG87880.1094217914967016No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT87000.10832607942891487No Hit
AGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC86660.10790273612999728No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA86640.10787783358300213No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG84760.10553699416545774No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT84650.10540003015698442No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA82800.10309654455993277No Hit
GGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC82790.10308409328643521No Hit
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA80320.10000862873253383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC1193550.0144.05257145
CGAAAGG263200.0143.23321
CGTGCCC267200.0142.92598145
AGTTGTC448650.0141.12611145
CGTACTG315000.0140.9605145
GGTCACA345350.0139.15233145
GGTCACC2607400.0138.39304145
CGTTGTC213900.0138.24353145
CGTTGCC54900.0136.14076145
CGAAAGT52800.0135.247271
CGATAGT25950.0134.379781
GGTGCTC223250.0133.20056145
CGATAGG36800.0133.175721
GCGATTC88100.0131.335831
AAAGTCC60350.0130.819183
ACCCCAA39100.0130.718958
ATAGTCC26100.0129.161543
GGTCATC71050.0129.0709145
AGTTGCC114900.0128.39445145
CGAACGG23300.0128.193941
Produced by FastQC (version 0.11.5)