FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443094
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC47271.0668165220021035No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT45221.0205509440434761No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA39190.88446243912127No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT35610.8036669420032769No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT33570.7576270497907893No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA33050.7458913909915278No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC30160.6806682103571703No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA28180.6359824326215205No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC27980.6314687176987276No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA27940.6305659747141691No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC26800.6048377996542494No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC24820.5601520219185997No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA22460.5068901858296434No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA22030.4971856987456386No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA21810.49222061233056646No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC21800.49199492658442673No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT20210.4561108929482232No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG17810.4019463138747083No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG17330.39111339806000534No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA17240.38908222634474854No Hit
GGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC16600.37463833859181117No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC16060.3624513083002704No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC15900.35884033636203605No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA15730.3550036786776621No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT14460.32634158891792714No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT14130.31889395929531883No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT13800.3114463296727105No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG13640.30783535773447623No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC13090.2954226416967957No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG13080.29519695595065604No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC13000.29339146998153887No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG12780.2884263835664667No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG12070.27240269559055186No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA12070.27240269559055186No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA11380.25683037910691636No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA11200.25276803567640277No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT11080.250059806722727No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC10870.24532040605379446No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC10720.24193511986169974No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA10540.23787277643118615No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA10180.22974808957015894No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG9900.22342888867824887No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG9780.22072065972457314No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA9470.21372440159424408No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG9430.21282165860968552No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT9210.20785657219461331No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG9160.20672814346391508No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC8600.19408974168009496No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA8590.1938640559339553No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG8380.18912465526502278No Hit
GCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG8300.18731916929590559No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG8170.1843852545960902No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA8050.18167702564241447No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA7950.17942016818101802No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT7940.1791944824348784No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC7890.17806605370418016No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC7880.1778403679580405No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA7750.17490645325822513No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA7550.1703927383354322No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG7450.16813588087403578No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG7430.16768450938175647No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA7430.16768450938175647No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG7050.15910845102844995No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG7000.1579800222977517No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG6990.15775433655161206No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC6970.15730296505933278No Hit
GCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAATCC6960.15707727931319315No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC6950.1568515935670535No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA6920.15617453632863454No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC6850.15459473610565705No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG6790.15324062162881916No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA6700.15120944991356236No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC6700.15120944991356236No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC6670.15053239267514343No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT6630.14962964969058484No Hit
GTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC6560.14804984946760733No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC6180.1394737911143008No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC6180.1394737911143008No Hit
GGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG6060.13676556216062505No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC6020.1358628191760665No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC5940.13405733320694932No Hit
CTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTACCA5810.1311234185071339No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC5790.13067204701485463No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA5780.130446361268715No Hit
GGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG5720.1290922467918771No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT5480.12367578888452563No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT5450.12299873164610668No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT5410.12209598866154811No Hit
GCTTGCGAAATAAACATGAAATGGTTATTTATGAAGCTGCTTCAGCTATC5370.12119324567698954No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG5320.1200648169462913No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC5210.11758227373875521No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC5170.11667953075419663No Hit
CCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGCTGG5090.11487404478507947No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG5060.11419698754666054No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC5030.11351993030824159No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGA5020.11329424456210195No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG4950.11171444433912443No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA4930.11126307284684514No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC4930.11126307284684514No Hit
GCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA4910.11081170135456586No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG4860.10968327262386762No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG4860.10968327262386762No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG4850.10945758687772797No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG4840.10923190113158833No Hit
CCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG4800.10832915814702976No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT4770.10765210090861081No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT4770.10765210090861081No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC4570.10313838598581791No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA4510.10178427150898005No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA4510.10178427150898005No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG4500.1015585857628404No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG4500.1015585857628404No Hit
GGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC4500.1015585857628404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)