FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248712
Sequences flagged as poor quality0
Sequence length109-123
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1417793.3369877741772096No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT808901.9038711025835593No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT759941.7886361796233776No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT584201.3750049426743916No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG400560.9427798353948209No Hit
GGAGAGTACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCC370030.8709227643577631No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA364660.8582836398419098No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG329040.7744464675412219No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG276620.6510679001071383No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC276270.6502441210418592No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG271800.6397232855510093No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA231790.5455535701172497No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC213600.5027405952674598No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC213150.5016814507549582No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC202880.47750941932519786No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC188820.4444170374457012No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC178930.42113939471538664No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC174710.4112069728425933No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC161460.3800210510855996No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT158520.37310130693725535No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG156250.36775851128530246No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC149030.3507651259958312No Hit
GGCACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATC139420.32814650651773997No Hit
AGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCCTCTGCT138070.324969072980235No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG137630.3239334650124555No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT132490.31183568102521425No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT132090.3108942192363239No Hit
GGAAGTCTCAGAATGACCCCCTTGAGAGTACTGTTCCCCTATCACCGATG131970.3106117806996567No Hit
GACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCATCCTG131450.30938788037409926No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC124950.2940891263046307No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG124450.29291229906851773No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA119760.2818736595937781No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG113860.26798709820764505No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG111710.26292674109235925No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG111320.2620088158481912No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT109510.2577487012534622No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC105010.24715725612844552No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG103550.24372092059899564No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT102510.24127311994788067No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG100640.23687178608481815No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG92850.21853681774617814No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC92770.21834852538840005No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC92260.21714816160756484No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC91490.21533584766395084No Hit
GCAGGTCTCTCAAATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAG89810.2113817081506113No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC89810.2113817081506113No Hit
GCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGG89370.21034610018283187No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA87710.2064390337589368No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC86270.20304977131893148No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG85590.20144928627781783No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA85340.20086087265976135No Hit
CCATGATACTATGTATTGGTATAAACAGGACTCTAAGAAATTTCTGAAGA84790.19956636270003708No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG83470.1964595387966989No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA81440.19168162021808022No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC79510.18713906708668415No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC79350.186762482371128No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC79130.1862446783872383No Hit
GGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGG77930.18342029302056717No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT76840.1808548096458409No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA76790.1807371269222296No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT76120.17916017842583823No Hit
GAGAGTACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCA73340.17261701899305013No Hit
GAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCATAC69620.16386142435636963No Hit
TCCAATTTCAGGTCATACTGCCCTTTACTGGTACCGACAGAGCCTGGGGC68260.16066045427414238No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT67460.15877753069636164No Hit
GACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATCGG65710.15465863536996624No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG64170.1510340074827383No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA61310.14430255569217212No Hit
CAGGTCTCTCAAATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGG61010.14359645935050433No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG60260.14183121849633487No Hit
GATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCATACTGCCCTTTACTG59950.14110158560994485No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC58430.1375240308121614No Hit
GATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGCC57100.13439367036410094No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT57090.13437013381937868No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC54940.1293097767040929No Hit
GGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTG54160.1274739262157567No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC53830.1266972202399221No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG53390.1256616122721427No Hit
GACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTC53020.1247907601174191No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT52800.12427295613352941No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT52510.12359039633658389No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT52400.12333149434463904No Hit
GCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCC51310.12076601096991275No Hit
GGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCCTCTG50990.12001284153880046No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG50730.11940089137602171No Hit
GCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGA49200.11579980003351605No Hit
GGTATCGACAAGACCCAGGCATGGGACTGAGGCTGATTTATTACTCAGCT49070.11549382495212666No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA49020.11537614222851537No Hit
GAGTCTCAGAATGACCCCCTTGAGAGTACTGTTCCCCTATCACCGATGCA48640.11448175352906953No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA47150.11097480836545287No Hit
GGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTG47010.11064529673934125No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC46460.10935078677961699No Hit
GGATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCATACTGCCCTTTACT46320.10902127515350535No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC46290.10895066551933857No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC46260.1088800558851718No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT45710.10758554592544753No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG45630.10739725356766945No Hit
GAGAATGACCCCCTTGAGAGTACTGTTCCCCTATCACCGATGCACAGACC45530.10716188812044686No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG45210.10640871868933456No Hit
GAGGCAGGGAGAATGAAGTCTCAGAATGACCCCCTTGAGAGTACTGTTCC45090.10612628015266744No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG44970.10584384161600033No Hit
CCAGGATATGAACCATAACTCCATGTACTGGTATCGACAAGACCCAGGCA44860.10558493962405549No Hit
GCCCACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATC44440.10459640474572059No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC44430.10457286820099833No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA44030.10363140641210795No Hit
GGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT43770.1030194562493292No Hit
GTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC43240.10177201937904946No Hit
ACAGAATGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGT43160.10158372702127139No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT42830.10080702104543683No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC42580.10021860742738034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGT59300.0109.915931
GACTGTT15300.0109.644361
GGATCTG42450.0108.264131
GTCTTTC12950.0107.501221
GAGTGAT17550.0106.8720251
GGCTCAT40250.0105.7880861
GGAAGTC16600.0105.26851
GGACGTG9250.0105.162281
AGTCATT6750.0104.4233254
GGATTGG3200.0103.755261
GCTTCAA4050.0102.114781
GACTTCG750.0100.9641951
GCTATTT4300.0100.241721
GGTTCAC7150.099.389171
GACTGCA2700.099.2383041
GCCCGAC2800.097.774391
GGATCCT25400.097.004511
GACTACT10750.096.990651
GATCCTT6250.095.9936451
ATTGAGG3400.095.948988
Produced by FastQC (version 0.11.5)