FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5354135
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1430252.6712998458200996No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT590491.1028672231835768No Hit
CAATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGC535210.9996199199310438No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA520940.9729676222209562No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT350010.6537190414511401No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC336550.6285795931555704No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT333370.6226402584170926No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC309130.5773668389011484No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG277340.5179921686696357No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG250180.4672650204001207No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG237240.4430967840743649No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT207970.388428756465797No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA204550.3820411700489435No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG193310.3610480497783489No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA174720.3263272218574989No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC174420.3257669072595293No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA153210.28615266518307814No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA150330.2807736450425699No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT146720.27403119271366894No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG146690.273975161253872No Hit
CCTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG137370.2565680544102829No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG133300.24896645303116188No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC131760.24609017142825126No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT131040.2447454163931242No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC130640.24399833026249804No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA126520.2363033431170488No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC123890.23139125180818193No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA122420.22864571027813083No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG120400.2248729253184688No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC112440.2100059113190086No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG111700.2086238019773502No Hit
CACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG111490.2082315817587715No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA109440.2044027653393125No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA109150.20386112789460856No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC108890.20337552190970157No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA106980.19980818563596175No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG106860.1995840597967739No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC102410.19127272659355807No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG96810.18081352076479207No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA94890.17722750733778658No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG93000.1736975253705781No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT92510.17278234486056104No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG91370.17065314938827655No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC88790.16583444384573792No Hit
GATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCA87460.16335038246140599No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG86390.16135192706198107No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC84630.15806474808722604No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC82940.1549083091853306No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC81890.15294720809243698No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT80650.15063124108749593No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG80600.15053785532116765No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG80000.14941722612522845No Hit
CCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA77440.14463587488922114No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG76740.14332847416062539No Hit
CCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA75860.14168488467324786No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC74770.13964907496729165No Hit
GGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG73920.13806151693971108No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA73390.13707162781663146No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG71490.13352296869615726No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC69710.13019843541487094No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT69620.13003034103548006No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC68820.12853616877422777No Hit
CCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC67730.12650035906827153No Hit
CTCAGCAGAGATGCCTGATGCAACTTTAGCCACTCTGAAGATCCAGCCCT67530.12612681600295847No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT66980.12509957257334753No Hit
GGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG66470.12414703775679918No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG65600.12252212542268734No Hit
GATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAG63850.11925362360119796Illumina RNA PCR Primer (96% over 27bp)
TGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGCTA63620.11882404907608791No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA62930.11753532550075783No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA61230.11436020944559672No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA60180.1123991083527031No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT59360.1108675817849195No Hit
TTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATC58960.11012049565429335Illumina RNA PCR Primer (96% over 30bp)
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT58900.11000843273469944No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC57460.10731892266444532No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA55610.10386364931029943No Hit
CGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG55290.10326598040579851No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTAATGATAC55240.10317259463947025No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC54540.1018651939108745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)