FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5990235
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC1304962.17847880759269No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1090171.819911906628037No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA815731.361766274611931No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG729261.2174146757180644No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA685601.144529388246037No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC578770.9661891394911885No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA560070.9349716663870449No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC478480.7987666594048479No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT451480.7536933025165122No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA354390.5916128499132337No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC315500.5266905221581457No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA314470.5249710570620352No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC308730.5153887952642927No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT256840.4287644808592651No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC252730.4219033143107074No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA239580.39995092012249933No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT239400.3996504310765771No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA229850.3837078178068139No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA229350.3828731260125855No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG214730.3584667379493459No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG197580.32983680940731036No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA192550.32143980995737226No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA191580.3198205078765691No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC190460.3179507982574974No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC182840.305230095313456No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC180330.30103994250642924No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC176450.2945627341832165No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT174270.29092347796038054No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC173410.2894878080743076No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA167170.2790708544823367No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA163750.27336156260981415No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT161750.27002279543290036No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC159250.2658493364617582No Hit
GCCCGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTC158620.26479762480103036No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT157760.2633619549149574No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG155900.2602569014404276No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG155570.25970600485623685No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG155250.25917180210793067No Hit
CCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC150140.25064125197091597No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC149120.24893848071069No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT146620.24476502173954778No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT144000.24039123673779075No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC142100.23721940791972268No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA138480.23117623932950876No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG137710.22989081396639696No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG133000.22202801726476507No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC126410.21102677941683426No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC119230.19904060525171383No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT118800.1983227703086774No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG116520.19451657572699568No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA116520.19451657572699568No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA111410.18598602558998104No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC110540.18453366186802356No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG109300.18246362621833703No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT108740.18152877140880117No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG105280.17575270419274033No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA104430.174333728142552No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA104070.17373275005070754No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA103130.17216352947755806No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA102330.17082802260679256No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG98180.1639000807146965No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT98020.16363297934054338No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC96730.16147947451143405No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC95790.15991025393828456No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC95740.15982678475886172No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA95460.1593593573540938No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC94250.15733940321206097No Hit
TTCTGTCAACTTCAAGAAAGCAGCGAAATCCGTCGCCTTAACCATTTCAG94240.1573227093761764No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA92430.15430112508106944No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA91030.15196398805722983No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT90390.15089558256061741No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG88900.14840820101381666No Hit
CAATGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGA88850.14832473183439382No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA88640.14797416128081786No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC87980.1468723681124363No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC84900.14173066665998912No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA83880.14002789539976312No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG83260.13899287757491985No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC82810.13824165496011426No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA81760.13648880219223453No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA81570.1361716193104277No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC81210.13557064121858325No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG80200.1338845637942418No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA78970.13183122198043984No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA78920.131747752801017No Hit
CCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA78630.1312636315603645No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC77820.12991143085371443No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC76930.12842567945998778No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG74930.12508691228307403No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC72790.12151443140377631No Hit
TGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC71820.11989512932297315No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA70930.11840937792924652No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT70890.11834260258570824No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA70300.11735766626851868No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG70300.11735766626851868No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC68640.11458648951168027No Hit
CCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA67420.11254984153376286No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC67100.11201563878545666No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC63770.10645659143589524No Hit
CCTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG62770.10478720784743838No Hit
CTATGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGT61430.10255023383890616No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT60240.10056366736864246No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC59920.10002946462033627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA780300.0144.03575145
GGTCACA996400.0143.60165145
CGTACTG378450.0143.32288145
AGTTGTA527400.0141.4104145
AGTTGTC162100.0140.30371145
GGTGCTC615050.0139.97667145
CGTGCTC161200.0138.79623145
AGAGCGA178500.0138.528888
GGGTTGC133200.0138.458569
GAGAGCG177050.0137.782147
CCGATTC70150.0137.779111
CATCCTA167850.0136.36012145
AGTGCTC729350.0136.0193145
CGTTGTA222350.0133.15707145
ACCCCAA47550.0132.786478
TTGACCA109450.0132.57639145
CGTTGTC83150.0131.14355145
TGTTGTA51050.0130.48973145
GAGCGAT186900.0129.903069
AGGGTTG142250.0129.138588
Produced by FastQC (version 0.11.5)