FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6711413
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT656660.9784228745869163No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG594650.8860280242029509No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC506150.754163095014418No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG441040.6571492471108543No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC331160.4934281350290915No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG278590.415098877091903No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT262180.39064799022202923No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT254030.37850449674308523No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC243790.36324690493641204No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT240130.3577935078648863No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA230910.3440557152420809No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC225760.3363822193627482No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG222650.33174832185115116No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC218370.3253711252757057No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG216300.32228682693197397No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA203010.30248473756569594No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG194250.28943234457483097No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC185740.2767524513839336No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT183550.27348935313621736No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA176350.26276135889715024No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC175990.2622249591851969No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC163910.24422576885076214No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC163010.24288476957087876No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG160520.23917467156320138No Hit
CTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG159370.23746117248335039No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA151890.22631597846831955No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT142230.2119225861975712No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG138670.20661818904603246No Hit
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA137200.20442789022222296No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG132900.1980208936627801No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA131780.19635209455892522No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC123250.18364240138403046No Hit
GAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGG119200.17760790462455522No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG118030.17586460556070682No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC113390.16895100927330803No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA112680.16789310984140002No Hit
CCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG112080.16699911032147777No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG111070.16549421112960863No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA110170.16415321184972523No Hit
CGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG107810.16063681373803101No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA105430.1570906156423394No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC105400.1570459156663433No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT105370.15700121569034717No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA101010.1505048191789121No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA98460.1467053212192425No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG95860.1428313232995794No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA87910.13098582966060945No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT85470.1273502316129256No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC82120.12235873429335968No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG76270.11364223897411765No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG73680.10978314104645326No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG70940.10570054323880827No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA67420.10045574605526436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC1025400.0143.4693145
CGTACTG298800.0142.66019145
CGTGCCC367850.0142.24995145
CGTTGTC283850.0141.899145
AGTTGTC229400.0141.44992145
CGTACCG116150.0141.36926145
GGTCACC1345100.0139.83693145
CGTGCTA170150.0139.74875145
CGTTGCC125900.0138.25232145
GGTGCCC102600.0137.99422145
CCGATTC74700.0137.137651
GGTGCTC311050.0135.34045145
CGTGCGC94700.0132.81757145
AGTTGCC99200.0131.6158145
CGATCGG54550.0131.309861
TGTTGCC27800.0128.82144145
ATCGGTT82450.0128.376163
CCGGTTC31550.0127.764611
ATTGACA29550.0126.843492
CGTGCTG53100.0125.6028145
Produced by FastQC (version 0.11.5)