FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6546413
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA775501.1846182023651732No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA772101.179424518434752No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC671521.0257831273401175No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA643960.9836837364217625No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT620260.9474807043185328No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT607390.9278210830877918No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA599680.9160436409985132No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA559610.8548345483244031No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG470250.7183323142001582No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG433150.6616600571946806No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG392680.5998399428816972No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC331260.5060175702327366No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC325510.49723413417393614No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA319840.4885729024429104No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG305170.4661636838372403No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA303170.46310857564287494No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC302730.46243645184011456No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG289130.4416617161184301No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC288630.4408979390698387No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC288120.4401188864802756No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG283480.4330310354693479No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG262350.4007538173958777No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA240830.3678808532245063No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT240790.367819751060619No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG234190.3577378940192133No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC225640.3446773064883013No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC223800.3418666069494852No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA222410.33974330675440123No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT209070.3193657350979842No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA207970.3176854255910832No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC204320.31210985313636647No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA202680.30960466441698686No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT198690.3035097235692279No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT196690.3004546153748625No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA195590.2987743058679616No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG193410.2954442379361033No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC179240.27379879637902466No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT170650.2606771066842254No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG170010.2596994720620285No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT165750.25319209160803025No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA162130.2476623457762289No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT160780.2456001477450323No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC158950.24280472374718798No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC156840.2395815846021325No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC151810.2318979874933036No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC144820.2212203843539966No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG142760.21807362291380028No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC142150.21714181491451884No Hit
CGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC139250.21271190803268902No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC138430.21145931367299925No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT136650.20874026738001406No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG132820.20288973518780437No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT128640.19650455906158074No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA127380.19457984089913055No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG124080.1895389123784277No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA124020.18944725913259672No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA122930.18778222516666762No Hit
CCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC122550.18720175460973817No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA119410.18240523474458456No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC118720.18135122241752852No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG116470.17791422569886745No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA115950.17711989756833246No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG113850.1739120339642488No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC112380.17166652944139027No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG112020.17111660996640451No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG111600.17047503724558777No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC111450.17024590413101037No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT109830.1677712664935744No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC103700.15840735987784454No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG103690.1583920843368727No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC101740.1554133538473665No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC101700.1553522516834792No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA100320.15324422702936707No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT99520.15202218375162094No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC98710.15078486493290297No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA98190.14999053680236796No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT96710.14772975673853758No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC96580.14753117470590382No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT96290.14708818401772084No Hit
TGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA93750.14320819661087683No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT91520.13980175097415942No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA90910.13886994297487798No Hit
GGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC90140.13769372632004734No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG89580.13683829602562503No Hit
CCGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC87990.13440948501110456No Hit
GGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA87880.13424145406041446No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG86680.13240838914379524No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG86320.13185846966880946No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG83530.12759659373766977No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG83310.12726053183628958No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA82500.1260232130175716No Hit
GCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG81820.12498447623148737No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC81460.1244345567565016No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC80290.12264731846279787No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC78440.11982134338300991No Hit
GAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG77120.11780497197472876No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA74950.11449017958384232No Hit
CTTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC74570.11390970902691291No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC72490.11073239650477291No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC71210.10877712726037908No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA70600.10784531926109765No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA70440.10760091060554842No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA69580.1062872140819713No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA68300.10433194483757746No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA67770.10352234116607065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA968050.0143.86589145
CGTACTG422950.0143.3532145
GGTGCTC689250.0142.27664145
GGTCACA985850.0142.12027145
CGTGCTC193900.0139.1698145
CATCCTA274000.0136.51088145
AGTTGTA313700.0136.22227145
CGTTGTA220700.0135.42561145
AGTGCTC830700.0133.39899145
CGTGCTG123650.0131.98457145
GGGTTGC85450.0131.590999
AGTTGTC97050.0130.48413145
CCGATTC50000.0130.31551
TGTTGTA74050.0130.04391145
CTTTCCA46250.0127.90093145
CGCTAAG40650.0124.8478551
CCGATTT26650.0124.420481
CGTTGTC85900.0122.97746145
TTGACCA81050.0121.04579145
AGTGCTG199400.0118.48281145
Produced by FastQC (version 0.11.5)