FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799522
Sequences flagged as poor quality0
Sequence length110-124
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2223342.5266599708484163No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC1855382.1085008935712644No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1165301.324276477745041No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT875560.9950085925121841No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT852000.968234410914593No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG623940.7090612421902007No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT569880.6476260869624508No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC535570.6086353326919348No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT512870.5828384769081776No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG505820.5748266780854687No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC449920.5113005001862602No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG405920.46129778412963796No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA403310.4583317139271883No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT398590.45296778620475064No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG391920.4453878290207127No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT388470.4414671615117276No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC379300.43104614091538157No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT377890.42944378115083975No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC371790.42251158642480807No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC367690.41785224242862284No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT365320.41515891431375473No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC358840.4077948779490522No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG347510.39491917856447206No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT340790.3872824001121879No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG322520.3665199086950405No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC321320.36515619825713264No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG314550.35746259853660234No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT307500.3494507997138936No Hit
AGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGAC290340.3299497404518109No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC262410.29820938000950503No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG261350.2970047691226864No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC256260.29122036401522716No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA247410.2811629995356566No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC241600.27456036816545265No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT238960.2715602052020553No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT236450.268707777536098No Hit
ATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGC234180.26612809195772225No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT231760.26337794257460806No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT212690.24170631086552202No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC204490.23238762287315154No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC190610.21661403880801708No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT186130.211522853173161No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG180960.2056475340365079No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA178960.20337468330666142No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC174870.19872670356412542No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG172200.1956924478397804No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC168640.19164677354065368No Hit
AGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAG168450.19143085272131827No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG167470.1903171558636935No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA164490.18693060827622227No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC163390.18568054037480672No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG162760.1849645923949051No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC161450.18347587516685565No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC160220.18207807196800008No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC155040.17619138857769776No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT153240.17414582292083594No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC150470.17099792465999858No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC149710.17013424138265693No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG146430.16640676618570874No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG145620.16548626164012092No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG141770.16111102398516647No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT135350.15381517314235932No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG133380.15157641517346054No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC132530.1506104536132758No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC130830.1486785304929063No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG129480.14714435625025996No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT121820.138439337954948No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT121270.13781430400424022No Hit
GGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGG120360.13678015692216008No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC119720.13605284468860923No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC118640.13482550529449214No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT118560.13473459126529827No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG115360.13109803009754395No Hit
AGTGTCAAGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAA114520.13014343279100843No Hit
GACAGACCCAGAAGACCTCTCTGTCTTGTAGCATCTGCCATGAGAATCAG111840.12709781281301416No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC111070.12622276528202325No Hit
AGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACAC110570.12565455259956165No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT110110.12513179693169696No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT109900.12489314760506309No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT108690.12351807291350599No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC104900.11921102078044693No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC101530.11538126730065565No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA100050.11369935776056926No Hit
GAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTC99910.11354025820948001No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG99630.11322205910730151No Hit
CAACATCTGGGTCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAA97020.11025598890485187No Hit
TGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGCA96730.10992642554902414No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC95240.10823315175528853No Hit
CTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCC93380.10611940057653133No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG92600.10523298879189119No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT92180.10475569013862343No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG92060.10461931909483266No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC91500.10398292089047564No Hit
ACCTCCCTGACGATCCAGTGTCAAGTCGATAGCCAAGTCACCATGATGTT89800.10205099777010615No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG88300.1003463597227213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTTC255100.097.37011
GCTTCAA42100.096.024171
GAATGCG13600.095.32841
GTTAGTG5200.095.1950761
GTCTTTC13950.093.3590851
GCTATTT10450.090.7921141
AGTCATT33500.088.8336944
AATGCTT59100.088.7429054
GAGAACT39050.088.433151
AGTGGTA584700.088.4090352
GAGTGAT51650.086.8266141
GAGTTAT251800.086.11518118
TGCTTAC60850.086.093886
GCCTGTT16350.085.782311
CTTACTA61300.085.556068
GATCACT42150.084.86521
TTACTAC61600.084.851929
GGATCTG61200.084.447481
GACTTCG6000.083.4845661
ATGCTCA130550.082.559672
Produced by FastQC (version 0.11.5)