FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6564299
Sequences flagged as poor quality0
Sequence length111-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2906084.427098765610768No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT1861172.8352913235670707No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1270751.9358502712932484No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC1020301.5543167671064344No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT821631.2516644960870915No Hit
GACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCA738981.125756154617576No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC667991.017610562833899No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG654200.9966029883769767No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT612550.9331537152710443No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT557880.8498698794798957No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG497120.7573085869488883No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG473920.721965894606568No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC458700.698779869716477No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT366540.5583840711704326No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG354260.5396768184995838No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC300360.4575659944801418No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT294150.4481057307109259No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT289990.44176842035988917No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT269170.4100514007664794No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG258420.3936749377199302No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG249460.3800253461946203No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT238480.3632985030084705No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT236470.3602364852667436No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC230150.350608648387284No Hit
GGCTTCTGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTG219330.3341255479069433No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG204650.31176215464895796No Hit
GTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCC202330.30822788541472596No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC196410.29920940529978907No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT193850.29530952200684335No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA186230.28370127564268477No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC181490.27648039798309004No Hit
GATCCTGGCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGTGGTC175140.2668068593462912No Hit
ACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATG172040.2620843444212398No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA171610.2614292858993778No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC171240.2608656308921943No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT169720.2585500751870078No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG148790.2266654824833543No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC145900.22226287985967733No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG145520.2216839909333807No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC142260.21671773330252017No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG137520.20949685564292547No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA133690.20366226462262002No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA131990.2010724983733983No Hit
GCCTTATCCTCTGCTTCTGTTCACAGTGACACTGATCTGGTAAAGCCCCC131890.20092015918226758No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC131700.2006307147191193No Hit
GTTCTGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGAC130690.19909208888869934No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT129010.1965327904777037No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT128940.19642615304391225No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG128250.19537501262511048No Hit
AAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCATGT128170.19525314127220592No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTA125750.19156653284684322No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC123570.18824553848019415No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT122630.18681355008356568No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC121460.18503118154733658No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG119610.1822129065114188No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT118210.18008015783558914No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT115740.17631737981466108No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC112920.1720214146247756No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT110130.16777115119222935No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC109300.16650673590584464No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT106350.16201272976748926No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC105880.161296735569175No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG102230.15573635509290482No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG99850.15211068234399439No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG99790.15201927882931598No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC99420.15145562382213243No Hit
GTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGC93100.14182778694267278No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG91820.13987784529619995No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT89780.13677012579713385No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC88240.13442410225372123No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG85440.13015860490206188No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC83940.12787351703510155No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG83590.12734032986614413No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT83330.1269442479692043No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC83310.12691378013097818No Hit
ACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAG82180.12519234727120138No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA82090.12505524199918375No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTC80560.1227244523748842No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC79370.12091161600042899No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG79190.12063740545639375No Hit
GAAGGACGGCCCTTATCCTCTGCTTCTGTTCACAGTGACACTGATCTGGT78140.1190378439495215No Hit
GCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCA77960.11876363340548625No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC76740.11690509527369182No Hit
GAGAGCCCAGCACCTCGCCCAAAGGACCCCAGTCAGAGGCCCCATCTCAG76020.11580825309755086No Hit
GACACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCC73830.11247202481178874No Hit
GATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGCT73660.11221304818686657No Hit
GATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCATGTATCCCTTTTTTG71540.10898345733489592No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT71320.1086483111144084No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC68960.10505310620372411No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC68900.1049617026890457No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA68700.10465702430678431No Hit
GATCCTCTGCTTCTGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCC68540.10441328160097521No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA68380.1041695388951661No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC68090.1037277552408871No Hit
CAGACACCAAAACACCTGGTCATGGGAATGACAAATAAGAAGTCTTTGAA67800.10328597158660811No Hit
GAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCA67460.10276801833676374No Hit
TCAACTAGACAAATCGGGGCTGCCCAGTGATCGCTTCTTTGCAGAAAGGC67200.10237193643982397No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG66630.10150360305037902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG7500.094.308361
GGATCTG176050.093.645441
GCTTCAA32850.092.40641
TAGGTAA5850.091.18038118
GAGTCAG66050.090.578151
GAGAGCC19600.090.218451
GAGAACT74200.088.572971
CCGAGTT208.8065566E-488.4140852
GAGTGAT13650.086.362971
GGTTCAC99700.085.6651
GAGTTAT133050.082.623474118
GAGACCC129350.082.250951
CGGTCCA22500.082.020424118
GTCTTTC32900.081.874851
AGTCATT20600.081.8337254
ATTGAGG9250.081.563368
AATGCTT55550.081.278984
AGAATGC56750.080.182882
TTACTAC56350.080.0204549
GATCCTT8950.079.687931
Produced by FastQC (version 0.11.5)