FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6564299
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC1009001.5371024385086662No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC907331.3822191828860935No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC690901.0525114715219401No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC421590.6422467958878778No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT414160.6309279939868674No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG388950.5925232839028204No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG379360.5779139554733872No Hit
AGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC338560.5157595654920655No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG333920.5086910270236014No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC321880.49034938841146636No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA308180.4694789192265617No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC279410.42565093393826214No Hit
CGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC277090.4221166647040301No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG268530.40907642994324295No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG253800.3866368670896923No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG253170.38567713018556893No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC246850.3760492933061093No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC235460.3586978594363237No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC234410.3570982979294514No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA230820.35162932096785965No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA207670.3163627982211048No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC205240.312660955876629No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA201130.30639981512115766No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT197490.3008546685640005No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG177860.27095048534504595No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC175790.2677970640886407No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA171850.26179489995809146No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG170170.2592356015470959No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA155500.23688744220822358No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA152670.23257624309922503No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA152320.23204305593026764No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA152250.23193641849647617No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC151150.23026068739403857No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA144880.2207090201101443No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG143900.21921609603706352No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC139130.21194951662012956No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG132050.2011639018880767No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC131700.2006307147191193No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC131510.20034127025597095No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG130090.19817805374191516No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC128080.1951160360001883No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT127720.1945676149121178No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT126340.19246533407451427No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT122470.18656980737775655No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT119560.18213673691585347No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA113880.17348387085963027No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC113680.17317919247736888No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT110560.16842620971409134No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC109940.16748170672908103No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC109610.16697898739834977No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG104160.15867650148172716No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG103420.15754919146736004No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA102180.15566018549733948No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC101290.1543043666962763No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG100510.15311612100545696No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACAGAGC99520.1516079630132631No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG97780.14895726108758908No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC95050.14479840116972126No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG92720.14124889801637616No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA91990.14013682192112212No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG90910.13849155865691068No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC90540.13792790364972712No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA90310.1375775235101265No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC89170.13584085673123666No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC86010.13102693829150683No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC84670.1289855931303556No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA83630.1274012655425964No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG83340.1269594818883174No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG83160.12668527134428215No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC82700.125984511065081No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC82430.1255731952490281No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA82350.12545132389612357No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT80160.12211509561036145No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA79730.12146003708849948No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT78830.12008898436832326No Hit
AGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC74960.11419345767156554No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA74300.11318801901010297No Hit
GGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC73650.11219781426775349No Hit
CTCTGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCC73440.11187790196637905No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC72840.11096386681959491No Hit
CCCTGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGA72450.11036974397418521No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC72090.10982132288611472No Hit
TGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGG71650.10915103044513969No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA71320.1086483111144084No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA70670.10765810637205891No Hit
GCTCCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA70090.10677453906350091No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA67950.10351448037330414No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC67630.10302699496168592No Hit
GCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG67620.10301176104257287No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT67250.10244810603538931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC978400.0143.92058145
CGAAAGG266900.0143.498031
CGTGCCC191800.0142.0394145
CGTACTG266100.0141.228145
AGTTGTC372650.0140.4354145
CGAAAGT40700.0138.935761
GGTCACC2124200.0138.88551145
CGTTGTC188100.0138.66698145
GGTCACA294550.0137.85014145
CGAACGG17750.0137.640241
CGTTGCC47550.0133.70564145
GCGATTC62050.0132.490621
GGTGCTC180400.0131.96759145
CTAAAGT10750.0131.504331
GGTCATC63200.0130.30525145
CATCCTC184400.0130.24504145
ACCCCAA36250.0129.596768
CGTACCG52550.0129.39896145
ATAGTCC20350.0129.317143
AGTTGCC92850.0129.05998145
Produced by FastQC (version 0.11.5)