FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193456
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG4172295.092222378444456No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG2500393.0516914962379733No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA1788882.1833033581921963No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA1717932.0967098621143507No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG1266291.5454894735505993No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT540910.6601731918741005No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC468920.5723103901455016No Hit
TGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG424640.5182672610922667No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG418940.5113104897371756No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT332830.40621442282719283No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC313470.38258580994393576No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG286370.3495106338521864No Hit
TCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA283100.3455196439695289No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT273430.3337175423899268No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG259270.3164354577604371No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA256810.3134330617019241No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC251950.30750149875705685No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC243890.2976643799637174No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT241450.2946863936292573No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC239940.29284345946325946No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA237890.2903414627478319No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG236580.2887426258223636No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA234310.2859721221423536No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA220840.2695321729926908No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT216590.26434510663143856No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA215360.26284390860218204No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT196370.23966687561390457No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC196200.23945939295945445No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA188570.23014708323325347No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC181400.22139619716027037No Hit
GTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA180900.22078595405894655No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG179090.21857687403215442No Hit
GGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG176700.21565991200782672No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG175020.2136094951873788No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT160110.19541204590590344No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG160010.1952899972856387No Hit
GAGGACTGGGGAAGCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG159030.19409392080704407No Hit
GCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG152310.18589225352525235No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC148770.18157173236787993No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG144400.17623820766231002No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA135430.16529044642456125No Hit
GAGGACTGGGGAATCCGGCTCCACTCTGACGATCCAGCGCACACAGCAGG134720.16442390122068148No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG133510.1629471129154779No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC132300.16147032461027436No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC131750.16079905719881818No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT130450.1592124251353763No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA127790.15596593183633378No Hit
GAGGACTGTGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG125170.15276825798539714No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG122110.14903357020529553No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT122090.14900916048124258No Hit
TGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCACAG121080.14777646941656852No Hit
TGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAG119780.1461898373531267No Hit
GTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA119620.14599455956070306No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG118890.14510360463277036No Hit
GGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCACAG115500.14096615640579507No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA114100.13925747572208846No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC113480.13850077427644697No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA111040.13552278794198688No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG108530.13245936757334145No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG107320.1309825792681379No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA105920.12927389858443128No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC104640.12771167624504237No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA104140.1271014331437186No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTAATGATACGGCGA103900.12680851645508318No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC101930.12440415863586744No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG100710.1229151654686374No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG99210.12108443616466605No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA97910.1194978041012242No Hit
GCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA94050.11478672735900455No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC91950.11222370633344465No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTAATGATA91440.11160125837009437No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA90980.11103983471687649No Hit
GAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCTCACACAGCAGG89330.10902603248250799No Hit
TCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG89260.10894059844832266No Hit
CCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCCCTG88580.10811066783052231No Hit
GTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTG88520.10803743865836345No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC86240.105254730116327No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG86190.10519370580619461No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA85480.10432716060231483No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC85070.10382676125922931No Hit
GAGGACTGGTGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG83000.10130035481974885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC2116550.0144.35405145
CGTGCTA411750.0143.35031145
CGTGCCC423700.0142.8832145
GGTGCTC881250.0141.77122145
GGTGCCC169850.0140.80496145
CGTACTG271800.0140.61353145
AGTTGTC259900.0139.15807145
CGTACCG55150.0133.55168145
CGTTGTC186000.0132.71051145
GGGAATC745300.0132.550349
GGTGCTA96200.0132.25229145
ACTGGGG778900.0132.199165
GGTCACA144050.0131.70177145
TTGACCC76650.0131.17935145
GGTGCGC69050.0130.49942145
GACTGGG791700.0130.473884
GGGGAAT756500.0130.461758
GGGTTGC83100.0129.90849
CGTGCGC76950.0129.72583145
TGGGGAA793200.0129.533287
Produced by FastQC (version 0.11.5)