FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7371521
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG4743346.434682882949122No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG2173252.9481703979409404No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA1355851.8393083327036577No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG1132341.5361008942387873No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA1100201.4925006657377764No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT688130.9334979850155755No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG432440.586636055164192No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC394690.5354254569714988No Hit
TGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG390180.5293073166311267No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT343690.4662402779562047No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG343630.4661588836279514No Hit
TCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA327980.44492852967521895No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA298740.405262360373117No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC292210.3964039443148843No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT284820.38637887621835443No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT275870.3742375555872391No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA270710.36723764335745634No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG256170.34751308447740975No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC254950.3458580664695929No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA228160.3095154989045002No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA227000.3079418752249366No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT221030.29984313956373454No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC213420.2895196255969426No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA207200.28108174690135185No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC206710.28041702655395No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA205130.2782736425766134No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC202050.27409540039294467No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG194050.26324282329250637No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT192850.26161493672744063No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT187340.25414022424951377No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG185680.2518883145011728No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA179310.24324694998494883No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG175520.2381055415836162No Hit
GGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG173850.23584006611389968No Hit
GAGGACTGGGGAAGCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG168810.22900294254062356No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG143440.19458670741085862No Hit
GAGGACTGGGGAATCCGGCTCCACTCTGACGATCCAGCGCACACAGCAGG140750.19093752836083625No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG139910.18979800776529024No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC139210.1888484072690019No Hit
TGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAG137730.1868406805054208No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG133230.18073610588642428No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC132030.1791082193213585No Hit
GAGGACTGTGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG131780.1787690762869698No Hit
GTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA130590.1771547554432796No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA130300.17676134952338873No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC128900.17486214853081203No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA127530.17300364470236196No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG126600.17174203261443602No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT125040.16962578007985055No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT121750.1651626577472953No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC121090.16426732013650913No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA120880.16398243998762263No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG119590.16223246193017696No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA118580.16086232407124662No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA118280.16045535242998019No Hit
GCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA117470.15935652899856081No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG107230.14546523030999978No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG106660.14469198419159357No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA105340.14290130897002126No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC101220.13731223176329554No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC100140.13584713385473635No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCC99530.13501962485082794No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG98450.13355452694226877No Hit
TGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCACAG98410.13350026405676657No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA97930.13284910943074027No Hit
GGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCACAG97670.13249640067497603No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG96990.13157393162143877No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG96530.13094990843816356No Hit
GAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCTCACACAGCAGG96020.13025805664801063No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC95500.12955263913648213No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC94820.1286301700829449No Hit
TAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC91730.12443836217790061No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG89780.12179304650966877No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT88720.1203550800438607No Hit
CCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCCCTG86580.11745201566949344No Hit
GAGGACTGGTGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG85940.11658380950145839No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC83660.11349082502783348No Hit
CGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA82990.11258192169567177No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG82630.11209355572615204No Hit
GCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG82000.11123891527949252No Hit
GATGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG77310.10487659195436057No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA74860.10155299021735134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC1996800.0144.40616145
CGTGCTA376800.0143.48277145
CGTGCCC401100.0142.83269145
GGTGCTC755200.0142.0557145
GGTGCCC147750.0140.7639145
CGTACTG245500.0138.72343145
AGTTGTC225350.0138.22801145
GGGTTGC79300.0134.217449
CGTACCG48350.0134.03844145
GGTCACA148150.0133.5335145
CGTTGTC146850.0132.34612145
GGTGCGC55150.0131.44461145
GGTGCTA81500.0131.19664145
AGTGCTC430150.0130.54068145
CGTGCGC72300.0130.24443145
GGGAATC844800.0130.218779
ACTGGGG877100.0129.634725
GGCAGAG264350.0129.477131
TGGGGAA879050.0128.745097
TTGACCC74750.0128.69096145
Produced by FastQC (version 0.11.5)