FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6439114
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC2134903.31551825297704No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC1552512.411061521818064No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA823141.278343573354968No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT767021.191188725653871No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA508290.7893787872058176No Hit
CCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC377090.5856240470350424No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG370630.5755916108955362No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT311580.48388644773178424No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC297500.46202008537199374No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGA297400.46186478450296115No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA292290.4539289100953951No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC244440.37961744426329463No Hit
CCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC224230.34823113863180555No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG220190.3419569835228884No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT216600.33638168232461796No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC207800.3227152058497489No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG202160.3139562368363101No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT194820.302557153049317No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC161710.2511370353126222No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC154230.2395205303089835No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA142600.2214590392404918No Hit
CCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC135190.2099512448451759No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG130840.2031956570422577No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT127910.1986453415796024No Hit
GCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC115970.18010241781710962No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC115520.17940356390646292No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATCTGAGCACCTTGGAGC113790.17671685887219887No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA108310.16820637124921223No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC101740.15800310415377022No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG97370.15121645617704546No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG95170.1477998370583282No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC93950.14590516645613044No Hit
GCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC92770.14407261620154574No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC90720.1408889483863774No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG85840.13331026597758636No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC79040.12274980688336935No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA78830.12242367505840089No Hit
CTTCTCAGCTACGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC78420.12178694149536723No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG78010.12115020793233354No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG77920.12101043715020421No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG77300.12004757176220207No Hit
CCAAGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACAGGACACGT76900.11942636828607166No Hit
CTGCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC76100.11818396133381082No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG75600.11740745698864781No Hit
GCCCAATGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTC75530.117298746380325No Hit
CGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA72980.11333857421999363No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA67610.10499891755294283No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT67370.1046261954672646No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA67340.10457960520655482No Hit
CGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT66650.10350802921022986No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG66640.1034924991233266No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG65920.10237433286629186No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA65590.10186183999848425No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA65170.10120957634854734No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC64620.10035542156886801No Hit
GCGCGAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC64480.10013800035222238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC882300.0143.46332145
CGTACTG189700.0141.78148145
GGTCACC905650.0141.73366145
CGTGCCC281600.0141.38742145
CCGGTTC99600.0141.13721
CGTGCTA162050.0141.09953145
GGTGCTC649450.0140.86145145
GGTGCCC154600.0138.84871145
CGTACCG67750.0138.0363145
AGTTGTC192750.0137.69504145
CATCCTC370600.0137.1278145
CGTTGTC127850.0136.76958145
GGTGCGC111450.0135.42961145
AGAGCGA166000.0133.986258
GGGTTGC67100.0133.324199
AGTGCTG170650.0132.88422145
TTGACCC54100.0132.1273145
GGTTCTT150750.0131.2883
CCGATTC47500.0131.258641
GAGAGCG166750.0131.035927
Produced by FastQC (version 0.11.5)