FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005299020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8098196
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT870141.0744862189060378No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG634950.7840635124168395No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT630250.7782597506901536No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA592600.7317679147306388No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA530520.6551088662215634No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT529650.6540345528806663No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC482920.5963303432023626No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA465590.5749305153888594No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT384480.4747724061013095No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC384050.47424142364546373No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA363570.44895184063216054No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG331650.40953565460752006No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA327330.4042011331906513No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT322170.3978293437205027No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG305820.37763966196915955No Hit
CCTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG269950.3333458464082618No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA264730.3268999663628788No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA261150.32247922870723306No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA256930.3172681915824216No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG255680.31572463793170724No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC254160.3138476766924387No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG246750.30469749065100427No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC244850.3023512891019185No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA244700.3021660626638328No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT238950.295065715870547No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC230460.28458189947489543No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC218120.26934393783504373No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC210520.25995913163870077No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC193970.23952248130324336No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG189650.2341879598863747No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC175790.21707303700725447No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA175140.21627038910888302No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG170760.21086177711678009No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA167820.20723133893030005No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG165250.20405779262443144No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT161860.19987167512369422No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG159660.19715502069843704No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC155630.1921786037285341No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC152010.18770847235606547No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC149180.1842138668908483No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG142880.1764343564912482No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA141730.175014287132591No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG139170.1718530892559281No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG134260.1657900105159223No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT133510.16486387832549373No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG131250.16207313332500226No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT130910.16165328673200796No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC126810.15659043075766504No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC125710.15523210354503647No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT120030.14821819575619063No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT117290.14483472615382487No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC113650.14033989792294482No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG109960.13578332754603617No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC109740.13551166210351046No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG109590.13532643566542474No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC108100.1334865197137733No Hit
GTAAGCGGGGGCTCCCGCTGAAGCCCCGGAACTGGGGAGGGGGCGCCCCG108090.13347417128456757No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC105180.12988077838570466No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC103420.12770745484549895No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC102110.12608981061955032No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC100820.12449686325201317No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA100300.12385474493331601No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG98530.12166907296390454No Hit
GCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG90290.11149396729839584No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCC90060.1112099534266644No Hit
CAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCT90050.11119760499745869No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG89790.11087654583811012No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG87470.10801171026238438No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG87250.10774004481985863No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG86980.10740663723130435No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG85540.10562846342568148No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC83660.10330695873500716No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA81880.10110893833638998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)