FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707352
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC849331.488133200825882No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA398350.6979594039407417No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC392650.6879722855713122No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC344260.6031869069929452No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC334520.5861211994634289No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG308110.5398475510184058No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA290850.5096058557453614No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT265290.464821514425604No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT254470.44586351078398534No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT243620.42685294336147483No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC238360.4176367604451241No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA212610.3725195151797191No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT207900.36426700157971686No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG203560.3566627746107126No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT197900.34674574128247215No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG184710.32363519895040643No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA180700.3166091735712113No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC167280.29309564225230894No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA154070.2699500573996487No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA152410.2670415281903061No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG141620.24813608832957912No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG125420.21975164664804273No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA125110.21920848757882816No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG118390.20743420065907972No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA113970.19968980360769756No Hit
CGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC112060.19634324289092386No Hit
CAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC109520.1918928427754237No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT108410.18994798288242953No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC103020.18050402358221468No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT100020.17524764549304125No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG99350.17407372105312588No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC91730.16072252070662543No Hit
CTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG89200.15628964185142252No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG88100.1543623032187256No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA81890.14348160057413667No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC81820.14335895175205596No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC80800.141571783201737No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG80710.14141409185906179No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA79570.1394166681851759No Hit
CGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG76390.13384490741065208No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT74710.13090133568071496No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG73390.12858852932147868No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG73120.12811545529345306No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACA73100.1280804127728586No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC72210.1265210206064038No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT72170.12645093556521483No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC71140.12464624575459864No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG69730.12217574805268713No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTCCACA66660.11679672114143301No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC66310.11618347703102945No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC65710.11513220141319477No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACACCC64540.11308221395841714No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC64380.11280187379366123No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA63810.11180316195671827No Hit
CGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC60940.10677456025140906No Hit
GGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG60820.10656430512784212No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA60390.1058108909350606No Hit
CGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA60350.10574080589387162No Hit
CTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTG60110.10532029564673775No Hit
CCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC59690.10458440271425347No Hit
CTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG59240.10379594600087748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC931700.0143.78168145
CGTGCTA145200.0142.49907145
CGTACTG222250.0142.09238145
CGTACCG80450.0141.66129145
CGTGCCC319200.0141.45242145
GGTCACC1318650.0140.11894145
CCGGTTC48350.0138.999021
CGTTGTC138600.0138.92792145
GGTCACA120500.0138.13686145
CGTGCGC82200.0137.49883145
AGTTGTC142950.0136.32321145
GGTGCTC354350.0135.82976145
AACGATT37700.0135.766253
GAACGAT40200.0133.815482
GGTGCCC117750.0133.54367145
AGTGCTC389150.0133.48235145
GGTGCGC51300.0132.27664145
GTCGATT43150.0132.059683
CCGATTT20350.0130.0341
CGATCGG23650.0129.362921
Produced by FastQC (version 0.11.5)