FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005299026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5071882
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC374500.7383846863945178No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC301220.5939018297349978No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT281540.5550996651735983No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA269080.5305328475701918No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC227680.4489063428526137No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC220470.43469071244165386No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT192380.37930693182530667No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA190840.376270583582189No Hit
CTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTACCA186400.36751643669943423No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT172120.3393612075359797No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA165450.32621027066481434No Hit
CCAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGCTGG160480.3164111467892983No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG157410.31035816685009626No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC155080.30576421139135335No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG130470.25724178914257073No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG129530.255388433721447No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA124220.2449189472468011No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT124080.24464291558833584No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA121100.23876738457243285No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG119230.23508039027721858No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC111000.2188536720688691No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC109570.216034205843117No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT99240.19566701275778894No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA97820.19286726307907004No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA97730.19268981415577097No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT94870.18705088170426679No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG93550.1844482974958802No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA93160.1836793521615842No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA80250.1582252899416824No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC79850.15743662806035313No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG78790.1553466740748306No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA74970.1478149531081362No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA74440.14676997611537493No Hit
CTCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCC73310.14454200630061978No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACACCC72560.1430632652731274No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT71000.1399874839359433No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG70750.13949457026011253No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG67060.1322191644048501No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA65910.1299517614960285No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT64970.12809840607490475No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG64170.12652108231224624No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG62280.12279465492296547No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC61450.12115818151920726No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG60970.12021178726161216No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT59540.11739232103586006No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG59350.11701770664222866No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG58750.11583471382023477No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC58620.11557839870880277No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG57420.113212413064815No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG57160.11269978284195097No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA56790.1119702706017214No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC55670.10976201733399948No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA55620.10966343459883332No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA54840.10812554393024128No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC54500.10745518133111141No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC53870.10621303886801782No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA53550.10558210936295442No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT53100.104694864746459No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC52500.10351187192446512No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG51980.1024866114787371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)