FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5998014
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC846201.4108003082353593No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC470430.7843096064797448No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA382830.6382612644785424No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC343360.5724561496522016No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA318940.5317426734915924No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA299350.49908186276324135No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT287940.48005889949573305No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT269070.4485984860988988No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG257850.42989229434942966No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC252400.42080595343725435No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG240810.401482890836867No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT229610.38281004345771785No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC226640.37785840446521135No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT205510.34263007722222727No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT196290.32725832250474907No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG190820.3181386372222539No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA182960.30503429968652956No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC167670.279542528576959No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA157690.26290368778732426No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT152050.25350057535710985No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA145220.24211347289286086No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG126670.21118656942114505No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC126500.21090314227342585No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA118950.1983156424776601No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA115880.19319728163355404No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA115090.1918801790059176No Hit
CAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC107780.1796928116539908No Hit
CGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG107680.17952608980239126No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC106820.17809228187863516No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG105720.17625834151104017No Hit
CTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG103260.17215698396169132No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT98770.1646711728248717No Hit
CGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC94260.15715201731773218No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG92890.15486792795081838No Hit
CTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG92430.15410100743346047No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC90440.1507832425866295No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG88800.14804900422039696No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC87460.14581493140896304No Hit
GGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG86220.14374758044912866No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG84490.1408632924164565No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC83130.13859587523470268No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC82980.13834579245730336No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT78470.13082663695016386No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG77500.12920943498964824No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA77280.12884264691612923No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG76570.12765892176977248No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG76110.12689200125241457No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG75600.12604171980925685No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG74860.12480797810742023No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA73740.1229406933695053No Hit
CAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG73360.12230715033342704No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA73140.12194036225990804No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA68670.11448789549340832No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT68610.11438786238244858No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC68570.11432117364180876No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC67670.11282067697741287No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA66650.11112011409109748No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACA66170.11031984920341967No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG65980.11000307768538053No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC63590.10601842543215137No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC62220.10373433606523759No Hit
CGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA62030.10341756454719844No Hit
CCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC61510.10255061091888082No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT61500.10253393873372087No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTCCACA61410.10238388906728127No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC61270.10215047847504191No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA61240.10210046191956204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC896550.0143.6628145
CGTACTG227950.0142.38908145
CGTACCG85550.0142.11575145
CGTGCTA139600.0140.94627145
AGTTGTC211300.0140.91408145
AACGATT40750.0140.551643
CGTGCCC324550.0139.97098145
CGTTGTC158050.0139.58432145
CCGGTTC50150.0138.204881
GAACGAT43200.0137.615232
GGTCACC1276200.0136.74852145
GGTCACA114800.0136.09262145
CGTGCGC79250.0135.84898145
GGTGCTC344500.0135.2955145
GTCGATT62950.0135.094833
GGTGCCC118600.0134.29956145
CGTTGCC69800.0133.46791145
AAATCGC11850.0133.375054
CCGATTT24050.0132.941311
AGTGCTC413650.0129.1706145
Produced by FastQC (version 0.11.5)