FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6216736
Sequences flagged as poor quality0
Sequence length108-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT2687934.323699767852455No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2222223.57457675539061No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1081231.7392245705785156No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG813481.308532323071142No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT774881.2464418627395468No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT628451.0109002537666067No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT518090.83337944541959No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT492610.7923933073561432No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC488210.7853156383028007No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG482030.7753747304051515No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT414860.6673276780612849No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG397590.6395478270269157No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA393710.6333066097707865No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG389590.6266793378390203No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG346680.5576559789574465No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC345410.5556131062988681No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC341170.5487928070292836No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT331280.5328841372707479No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT324310.5216724660657941No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG302660.4868471172010521No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC287240.4620431042913838No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT285970.4600002316328054No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT282030.4536625007077669No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG271520.43675652303716933No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA262650.42248858565009034No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC254890.41000615113783184No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC253170.4072394259624343No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC248320.3994379043922728No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC244710.3936309986462349No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA240950.3875828087279241No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG228780.36800661955083824No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG223120.35890216345040227No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG211400.3400498267901355No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA201460.32406072897417554No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA200840.32306342106211366No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC193200.31077401388767356No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC191430.3079268606548517No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC188880.30382502972621No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA181450.29187342039295217No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG176840.28445795349842745No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC175750.28270462184657674No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC168050.2703187010032274No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT167580.2695626772634386No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC167550.26951442042898394No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC164910.26526781899697843No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT157990.254136575849449No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT154700.24884440967092697No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT151800.24417958234031492No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG138420.22265703417355986No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT138050.2220618665486197No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG133340.21448554353924631No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT131630.21173490397533365No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT129020.20753655937778281No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC128350.20645882340829658No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC121820.195954919108677No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC121690.19574580615937368No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC120680.19412115939940186No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT119720.19257694069685444No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG119130.19162788961924715No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT111490.17933848244480705No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG110320.17745646590107733No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT109770.17657175726940955No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG109070.1754457644654687No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG108940.1752366515161654No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC107310.17261469684413172No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC106880.17192301555028233No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG106480.17127959109088758No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG105920.17037879684773488No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC105450.16962277310794605No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG102210.16441103498684842No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC100630.16186950837223907No Hit
GAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTC98330.15816981773071914No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC97810.15733336593350594No Hit
GTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACT96730.15559611989314007No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG95640.15384278824128933No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC94830.15253985371101492No Hit
GACTCTGCCATGGACTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCT93930.1510921486773767No Hit
GATTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGGA91740.14756939976219033No Hit
GACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATCGG90960.1463147220663705No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG89750.14436836307670134No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT89500.14396622278957963No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC89290.14362842494839737No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG88910.1430171717119723No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC87270.1403791314284538No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC86740.1395265940197557No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC86350.1388992551718458No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC84990.1367116120099036No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT84930.1366150983409944No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA82240.13228806885156455No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG82110.13207895590226124No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG81830.1316285587806849No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC81660.13135510338544212No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT78310.12596642353801094No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC78270.12590208109207146No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC77260.12427743433209967No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC75570.12155896599115676No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG73390.11805230268745528No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC73250.11782710412666711No Hit
GACTTATACTTCTATTGGTACAGACAAATCTTGGGGCAGAAAGTCGAGTT72540.1166850257112414No Hit
GACACAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATC72530.11666894009975654No Hit
GCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT70890.11403089981623798No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG69740.11218105449547801No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG69390.1116180580935076No Hit
GGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCCCTCTGTCTCC68040.10944650054305023No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG66160.10642240558389483No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC65700.10568246745559083No Hit
GGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGT65660.10561812500965136No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT65450.10528032716846912No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC65400.10519989911104476No Hit
GAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCT64240.10333396817879995No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC63060.10143586602358537No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA62280.10018118832776557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTG46150.0104.324591
GAGAACT51950.0103.9141541
GGCTTTC31100.0101.843561
GATGTTC324300.0100.2867051
GAAGCTA7900.099.5004961
GACATTA21750.097.7916951
CATTGAG12300.097.748497
ATTGAGG12250.095.789698
GATTACG13550.094.6950151
AGTGGTA144350.094.2600252
GCCCGAC2950.094.0443341
GAGTGAT14550.093.7480851
GATCCTT15050.093.705861
TCTGATA1900.093.24916116
GCCGACG251.993845E-592.476931
GGCAATA2750.092.476921
GGAGAGT30850.092.177161
GAAATAG1900.091.2601241
GACTTCG5850.090.896121
AGTCATT14250.090.860374
Produced by FastQC (version 0.11.5)