FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005299041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389487
Sequences flagged as poor quality0
Sequence length114-124
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1378712.558146999890713No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1173382.1771645427477604No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1124442.086358126478457No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1107002.0539988314286686No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG997661.8511223795511522No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG924231.714875645863883No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC674601.2516961261804693No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG641521.1903173715791504No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT595501.1049289106736875No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT548551.0178148680941248No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC523420.9711870536101117No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG504240.9355992509120069No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG436670.8102255372357332No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT423290.7853994266986821No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG388730.7212745851321286No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT346190.6423431395232979No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG337000.625291423840525No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC336030.6234916235997972No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT334180.6200590148932542No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT324590.6022651135442019No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC302990.5621870875651058No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC286770.53209145879747No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT279240.5181198136297573No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG278770.5172477454718789No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT277350.5146129863565865No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT274720.5097331156007984No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC273880.5081745257016113No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG239580.4445321048181395No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT230200.4271278509438839No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC216920.4024872868234027No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC211120.39172559466234913No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA204350.3791641022605677No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA197000.36552644064268086No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG194750.36135164626985833No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC194690.3612403184199164No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG192990.3580860293382283No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC191550.3554141609396219No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG186120.34533899051987693No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTA183740.34092298580551356No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT180410.3347442901337363No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC176720.32789762736230743No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC174240.3232960762313742No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC173440.32181170489881505No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC170600.3165421866682302No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC165440.3069679915732239No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT156230.2898791666071372No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG155890.28924830879079955No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT153790.2853518340428319No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA151160.2804719632870438No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA149810.27796708666335035No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG146080.2710462053252935No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA143880.2669641841607559No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG139450.25874447790670985No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC139330.25852182220682596No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG138680.25731577049912174No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT136350.2529925389930433No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC131640.24425330277260157No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC131440.24388220993946177No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC130320.241804090073879No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG129820.24087635799102955No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT128900.23916933095858658No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG128860.23909511239195866No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC126570.23484609945250817No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT126380.2344935612610254No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT125530.23291641672018135No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC122340.2269974860316019No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG121380.22521624043253097No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA120060.22276702773380846No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA118700.22024359646845795No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT115510.21432466577987846No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG111780.20740378444182161No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG108360.20105809699513147No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT107200.19890575856292073No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT104610.19410010637376063No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT102980.19107569978367142No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC102580.19033351411739188No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT99110.18389505346241675No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC98870.183449742062649No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT94870.17602788539985345No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC94190.1747661697671782No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT93820.17407964802586962No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG91580.16992340829470412No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG90850.16856891945374394No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT90580.16806794412900522No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT88730.16463533542246228No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC86760.16098007101603548No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC84430.15665683950995707No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC84420.1566382848683001No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG81800.151776968754169No Hit
GGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGG81450.1511275562961744No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTC81270.15079357274634858No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG80700.14973595817190022No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG80600.14955041175533035No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC80070.14856701574750994No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC80050.14852990646419595No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT80020.14847424253922498No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC79970.14838146933094004No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG79440.14739807332311963No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG78290.1452642895325659No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG77690.14415101103314656No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC77550.1438912460499487No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT77210.1432603882336111No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA76640.14220277365916273No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT75960.1409410580264875No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT75900.14082973017654557No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC74780.1387516103109628No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA74680.1385660638943929No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG74330.1379166514363983No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC73430.1362467336872693No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT72640.13478091699636718No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA69800.12951139876578235No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC68690.12745183354185657No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT68640.12735906033357164No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT68280.12669109323392005No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC68150.12644988289237918No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC67810.12581902507604156No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC67310.12489129299319213No Hit
GATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAG67270.12481707442656416No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA67050.12440887231011041No Hit
ATTCTGTGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCAC66000.12246063493612656No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT65700.12190399568641691No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG65670.12184833176144595No Hit
GCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCC65660.12182977711978896No Hit
GATCCTGCCCTGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCC65590.12169989462819002No Hit
GGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAAT65300.12116181002013734No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC64190.11910224479621159No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA63760.11830439520496107No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT63440.11771064667193741No Hit
GGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC63070.11702412493062883No Hit
GAGAGACTCCAGGATCATCCACTGAGCACTGGACATAAGGAAGGCTGCAT61830.11472334936516221No Hit
GTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC61280.11370284407402782No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC60860.11292354912443428No Hit
GGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCC60240.11177316134170097No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT60160.11162472420844508No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC59910.11116085816702036No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC58850.10919406615137954No Hit
GGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGT58700.1089157465265247No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG58240.1080622330103032No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT58150.1078952412353903No Hit
GCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCA57390.10648508846945914No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG57180.10609544099466238No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTG57100.10594700386140647No Hit
GGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTC57100.10594700386140647No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT56580.10498216249524306No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT56380.10461106966210329No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT55240.10249584051320654No Hit
GAGTCCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGT54610.10132689808881624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)