FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6872565
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC3739225.440792484319901No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC2806474.083584513205768No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA959281.3958107344201183No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA561190.8165655763168482No Hit
CCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC536080.7800289993619558No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG525310.7643579944314822No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT491240.7147840726133547No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGA447900.651721737080697No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT413620.6018422524923372No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC366720.5335999004738405No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC349920.5091548788552746No Hit
CCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC349590.508674708787767No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA323320.4704502612925451No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT256100.37264107360206855No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC238490.34701745272689305No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT217330.31622836597398496No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATCTGAGCACCTTGGAGC193780.2819616838836737No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG192510.280113756654175No Hit
CCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC169990.2473457871988115No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG166990.2429806047669247No Hit
GCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC165670.24105992449689456No Hit
GCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC158230.23023427206581532No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC144640.2104599956493682No Hit
CTTCTCAGCTACGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC140620.2046106511906399No Hit
CTGCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC136920.19922692619131285No Hit
GCGCGAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC122470.17820129747772484No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC121780.17719730551839086No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG116960.17018391241115943No Hit
CCAAGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACAGGACACGT112160.1631996205201406No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC107950.1570738145073928No Hit
CTTCTCAGCTCAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC103420.15048238903524375No Hit
CTTCTCAGCTAAGATGCCTACTGCATCATTCTCCACTCTGAAGATCCAGC102830.1496239031569727No Hit
CTTCTCAGCTAAGATGCCTAATGCAGCATTCTCCACTCTGAAGATCCAGC99960.14544787863046768No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAGCACCTTGGAGC97730.14220309302276515No Hit
GCTCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC93880.1366011089018438No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT90660.13191581309161862No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG90190.13123193451062304No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG86380.1256881528221268No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC82660.1202753266065872No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT82260.11969330228233563No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC81970.11927133464725324No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC80970.11781627383662431No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA80840.11762711593124256No Hit
CGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA77610.11292726951291111No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA75260.10950787660793315No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT74770.10879489681072496No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA74290.10809646762162309No Hit
CTTCTCAGCTAAGAAGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC73950.10760174694600924No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG71520.10406594917618095No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC71140.10351302606814196No Hit
CTTCTCAGCTAAGATGCCTAATTCATCATTCTCCACTCTGAAGATCCAGC69310.10085026478469102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC931800.0143.32828145
GGTGCTC930950.0142.75827145
GGTCACC752550.0142.46928145
CGTGCCC284100.0142.31386145
CGTGCTA184450.0141.96683145
GGTGCCC205150.0141.74213145
CCGGTTC125250.0140.597321
CATCCTC565800.0140.39336145
CGTACTG137000.0140.28363145
GGTGCGC159900.0139.05388145
CGTTGTC110500.0139.02295145
AGTTGTC227950.0138.79152145
CAGCTAA755700.0138.288536
CTAAGAT716500.0138.243429
AGCTAAG747350.0138.0387
CGTACCG51350.0137.65182145
GCTAAGA752000.0137.396558
GGGTTGC51500.0136.968379
CGGGTAC111800.0136.631241
GGTTCTT206050.0136.375153
Produced by FastQC (version 0.11.5)