FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418618
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT764821.4114669090901037No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA661261.2203480666103423No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG333550.6155628612314062No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC308160.5687058951193829No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA286220.5282158661119865No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT271010.5001459781811525No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA258800.4776125572978202No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA202310.37336088279336166No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG199310.36782441574585995No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA195970.3616604824329746No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC193570.35723130879497317No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA189790.35025536031512094No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC186460.34410988189239394No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA169470.31275502351337553No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT146020.26947830609206996No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC144890.2673929035041776No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC143260.26438475640836834No Hit
CCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG138060.2547882135260319No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT131780.24319854250659484No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC130980.2417221512939277No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC120480.22234451662767146No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA120420.22223378728672144No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG119650.22081276074452932No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG107690.19874071211515554No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA101380.18709567642524347No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG100970.18633902592875157No Hit
CCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA97000.17901243453589089No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG93080.1717781175938219No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA92810.17127983555954673No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT92340.17041245572210478No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT90260.1665738385691702No Hit
CTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC82880.15295412963231583No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG75740.1397773380592616No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA75590.1395005147068865No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG72860.1344623296936599No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG72160.13317048738257614No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA70010.1292026859985332No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT68890.12713573830079922No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA67390.12436750477704832No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG66640.12298338801517286No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG66510.12274347444311447No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT66490.12270656466279778No Hit
CTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA65960.12172845548440579No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA65480.12084262075680553No Hit
CCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA65310.12052888762411375No Hit
CGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG62060.11453104832265348No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC60880.11235337128396945No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA60430.11152290122684418No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG59310.1094559535291102No Hit
CTTCTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCA58040.1071121824790011No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC57370.10587570483839236No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA55430.10229545614767455No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG54900.10131734696928257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC548350.0142.84242145
CGTACTG295850.0142.79202145
AGTTGTC329500.0141.01501145
CGTACCG101550.0139.64308145
GGTGCTC358250.0139.00737145
CCGATTC91900.0138.0541
CGTGCTA96150.0137.23111145
CGTGCCC194050.0136.2924145
GGTCACC768100.0135.18123145
GGTGCCC111800.0135.01108145
CGTTGTC230800.0133.40648145
AGTTGCC141950.0133.2506145
TTGACCC60650.0130.53362145
CCGATTT26650.0129.217961
GGGTTGC82000.0129.169169
CGTGCTG80150.0128.98717145
GGTCACA84250.0127.52895145
TGTTGCC22350.0127.15663145
TGTCTTG69300.0126.79435145
CATCCTC211700.0126.778625145
Produced by FastQC (version 0.11.5)