FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843210
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC40380.21907433227901324No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA35050.19015738846902958No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC26260.14246884511260247No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC21440.1163188133744934No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT19890.1079095708031098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA14200.077.5031362
CCCGAAT13350.077.0443341
CGAATAG13300.073.514053
AACGGCG850.066.682833
TGATCGC2100.060.9406051
CGAATAC3750.060.4590953
ACGGCGC950.059.668444
GTGAGTT33200.057.197236
GATCGCC2250.056.68042
GTCTATC10600.055.258985
TGTGAGT35500.053.4914975
TTCATAG32150.052.3065459
TGAGTTG36700.051.999887
AGTTGAA46500.050.0819249
CGCGAAT502.8637254E-547.3982471
CGTCATT5100.047.233673
GAGTTGA49550.047.1898468
CTGTGAG40350.045.422944
CGGCGCA1250.045.3480155
CTTTTCA47350.043.9952356
Produced by FastQC (version 0.11.5)