FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785503
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC55190.3091005727797713No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT53370.29890736671963025No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA30680.17182833072809175No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC29820.1670117608315416No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA26110.14623330232433102No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG24290.13604009626419No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT24010.134471910716476No Hit
TGATTGCTTTGTTTTCAATTCGAAGATGATTACATTGGATTCCATTCTAT21970.12304655886884537No Hit
ATAGGGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATC21560.12075028717397841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCC4200.069.741922
GTCTATC21700.067.931425
TGATCGC4350.067.535611
CGTCATT8000.066.7328953
CCCGAAT4650.061.140451
AGTCTAT25750.058.8984154
CTCGTCA9150.058.517761
CAGTCTA25750.058.3446963
CGCCATT5300.056.161735
CCGAATA5050.055.1965752
ACGGCGC602.4534529E-855.1216964
TCGTCAT9800.053.5116582
CGGCTCG451.5644539E-552.4997987
TCGCCAT5600.052.309374
TTCATAG30100.051.6451539
CTATCAT26100.050.689467
ATCATTG25650.050.6576969
GATTGCC11550.050.312352
TCCAGTC29850.049.844321
GGGAATC16400.049.8400044
Produced by FastQC (version 0.11.5)