FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788532
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT46840.26189075733618405No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC39130.21878277827849876No Hit
ATAGGGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATC23240.12993896670565583No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA21390.11959528820283896No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC19570.10941934502709484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCC2800.072.5617452
TGATCGC3250.065.64631
CCGAATA5300.064.188022
GTCTATC18800.064.09385
CGTCATT9000.061.949363
CAGTCTA21000.057.8243943
TCGTCAT9850.057.0831642
CCCGAAT5400.057.0690571
AGTCTAT21550.057.0111474
CTCGTCA9900.055.5525361
CGAATAG5550.055.337273
ATAGGGA18150.053.549861
GGGAATC18800.053.285834
TCGATCA2100.051.7538036
CGATCAA2550.048.186757
GAATCCT20950.047.8173566
CGCCATT4450.047.7844125
CTATCAT24050.047.554847
TTCGATC2100.047.253475
TCTATCA26150.046.4403156
Produced by FastQC (version 0.11.5)