FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640114
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC41080.25047039413113964No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT37620.22937429959136987No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC23430.14285592342971282No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA22000.13413701730489466No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG17450.10639504327138234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC13850.068.221235
CCGAATA5700.064.648592
CCCGAAT5700.059.8507841
CACGATA3750.059.0551994-95
GATCGCC3250.056.691842
CGTCATT6650.056.12353
AGTCTAT16950.055.186754
TGATCGC3450.052.188731
CGAATAG6200.051.8151253
CTCGTCA7400.051.2236481
CAGTCTA18400.050.3242843
TTCATAG24650.050.2215279
TCGTCAT7350.050.1356432
GTGAGTT23900.048.0338826
TCTATCA19500.046.5163846
AGTTGAA32000.045.478519
TGAGTTG27650.044.6016467
CTATCAT19650.044.244657
TGTGAGT26250.043.5537345
TCGCCAT4650.042.671284
Produced by FastQC (version 0.11.5)