FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191978
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC41930.35176823733323936No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT36660.3075560119398177No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG21700.18205033985526578No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA17680.14832488519083406No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC16290.1366635961401972No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA16160.13557297198438226No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT14360.1204720221346367No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC13540.11359270053641929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC15700.074.337135
CCGTCGG353.5255198E-667.501033
CCGAATA5050.067.367372
GATCGCC2900.066.802742
CCCGAAT4850.066.41561
AGTCTAT18250.066.2804644
ACGGCGC1150.065.7401354
TGATCGC3100.064.178641
AACGGCG1200.063.0009653
CTCGTCA5750.062.6107141
CAGTCTA19850.062.1281553
CGTCATT5900.061.6661953
CGAATAG4500.060.9009323
TCGTCAT6100.058.8697552
GAGCGGT1400.057.3758773
TCTATCA20250.056.0008546
CGAATAC1550.054.871813
TTCATAG16800.054.844599
GTGAGTT17950.053.4367526
TCCAGTC23100.052.9066961
Produced by FastQC (version 0.11.5)