FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283713
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC34630.2697643476384519No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT29370.22878945683341992No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG19320.15050092972494633No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC16610.12939029206684047No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC15950.1242489559582243No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA15170.11817283146622337No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA14590.11365468761319704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC13050.068.438695
CCGAATA6150.063.7679752
ACGGCGC900.063.0070534
CAGTCTA14850.061.0906033
AGTCTAT14900.060.8927234
CGTCATT5800.057.8402333
CCCGAAT6650.057.770611
CGAATAG5500.055.8406373
CGAATAC2300.055.467123
CTCGTCA6100.054.426671
AACGGCG1050.053.9997373
GTGAGTT17900.052.535216
TCGTCAT6350.051.3422662
TCTATCA16900.050.6106956
GATCGCC2450.050.142612
CTATCAT16350.049.4287387
TTCATAG19400.048.4788749
TGAGTTG19900.047.7357947
TCCAGTC19150.047.305131
GCGCACC1600.047.260817
Produced by FastQC (version 0.11.5)