FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984072
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC18290.1858603841995301No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA15360.15608614003853377No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT14770.15009064377403278No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC14060.14287572454048078No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA12400.12600704013527464No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC11020.11198367599118764No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT10220.10385418953084734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA4550.066.458842
CGAATAG4000.063.784913
TGATCGC1950.060.7287451
CCCGAAT4650.060.1018681
GTCTATC6850.060.0146065
CTCGTCA3600.059.2105261
CGTCATT3750.056.6976973
TCGTCAT4000.054.3352932
CAGTCTA8350.052.6236153
AGTCTAT8950.046.98894
GTGAGTT12650.046.3190356
TGAGTTG13850.044.0161867
GCTGATA7100.043.3654561
GATAAGC6850.042.769034
GATCGCC2800.042.1857872
CTATCAT9450.042.0068327
CGCCATT3050.041.830425
GATTGCC6500.041.432932
AGTTGAA16550.041.404029
AACGCAC6600.040.08928314-15
Produced by FastQC (version 0.11.5)