FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287916
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC16700.12966684162631725No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC16030.12446463899819554No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC14790.11483668189540312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA5500.068.708442
CGAATAG5250.065.6823
CCCGAAT6150.060.846211
CTCGTCA4500.055.788531
CGAATAC1400.050.6111263
TCGTCAT5200.048.1454542
CGTCATT5350.046.795583
GATCGCC2050.046.0849272
GTCTATC7700.044.174865
GTGAGTT15750.043.193196
TGATCGC2200.040.9083981
TCGGACC1050.040.5140760-61
CGTCGGA704.4746084E-640.4936184
ACTAAAG10650.040.0289731
AACGCAC7500.039.67912714-15
TGTGAGT17200.039.277245
CAGTCTA9000.038.8393553
GAACGCA7900.038.56995812-13
AGTTGAA20750.038.0246739
AGTCTAT9650.037.696134
Produced by FastQC (version 0.11.5)