FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242136
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC25280.2035203874615984No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT21530.17333045656836288No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC15340.12349694397392878No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA14390.11584882814764244No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA13770.11085742623996084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG3100.065.529493
CCGAATA4100.063.373672
GTCTATC10300.056.8763855
CTCGTCA4700.056.46131
CCCGAAT4700.054.4448241
AGTCTAT11850.050.6329084
CAGTCTA12050.049.7905243
CGTCATT5450.046.8087733
GTGAGTT15000.045.6692926
TCGTCAT5950.045.257222
TGAGTTG16450.042.223237
GAGTTGA19800.041.7611858
AGTTGAA20050.041.004819
AACGCAC7950.041.0026614-15
TCTATCA13600.040.99126
CTATCAT13100.040.3967747
TCCAGTC15850.040.0623361
GAACGCA8700.039.10170712-13
TGTGAGT17550.039.033585
TTGAACG9000.038.5865610-11
Produced by FastQC (version 0.11.5)