FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086419
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC27050.24898312713603132No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT21860.2012115031125192No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC15810.14552396451092994No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA14270.13134895468507085No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA12060.11100689512977958No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG11410.10502393643704684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC8600.062.629345
CCGAATA4350.061.9066162
CCCGAAT4600.060.7892951
CGAATAG4350.056.476213
CTCGTCA4200.054.1657641
AGTCTAT10900.050.2808844
CAGTCTA11200.050.1973043
CGTCATT4450.048.8370323
AGCGTCG601.5472215E-647.251058
TTCATAG15650.046.798169
GTGAGTT13650.044.9968576
TCGTCAT4950.043.9042
CTATCAT12000.041.7384227
AGTCAGG14650.041.600983
TGTGAGT14950.041.0840875
TGAGTTG15900.040.7131657
CCGTACC1306.91216E-1140.4096994-95
TCTATCA13050.039.8248026
ATCATTG13100.039.3157549
AACGGGA10750.038.6763786-87
Produced by FastQC (version 0.11.5)