FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932537
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC17120.1835852089514947No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA15360.1647119631714345No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC12420.1331850639706521No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT11760.12610759680312952No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA9460.10144369606782358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCC1650.080.165952
CGGCGCA255.4170225E-575.593135
CGAATAG3000.070.860973
CCCGAAT3200.069.563731
CCGAATA3400.068.082112
TGATCGC2050.064.69031
CGCCATT2300.057.5165185
TCGCCAT2350.056.2927634
AAGCGCA1250.052.909522
GTGAGTT14050.052.457876
GTCTATC6200.051.817875
GCGCAGC1300.050.8799934
AGCGCAG1350.048.9902953
CGTCATT3350.047.9457243
TGAGTTG15650.047.3991937
CTAGGCG502.9195353E-547.2406433
CTCGTCA3350.046.6556361
TTCATAG14900.045.0285269
CGAATAC1052.1827873E-1144.9910933
TGTGAGT16200.044.3293085
Produced by FastQC (version 0.11.5)